164
edits
Changes
updated
==Protocol==
*Use the following volumes per reaction
::*Buffer, 12.5 uL of 10X buffer (Dave's fridge)DreamTaq::*Primers, 10uL 7.5 uL RNAse-Free water::*5.0 uL Working stock Primer (of 0.4-1uM stock solution in water (both primers combined)::*dNTPs, 5uL of 2 mM ("molecular biology stuff" box in freezer)::*Sterile water, 28 uL::==PCR Program==*Template 1 uLFor animals::*Polymerase 1 uL (turbo pfu found use program denoted in "enzymes" box in freezer)[[Genotyping Program]]
*Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''DAVETD''') as follows:
