Changes

*Primers – order from IDT prediluted to 100 mM in TE. Make working solution of 0.4uM-1 uM (100x) in water with both sense and antisense primers combined (300uL water, 3uL of each primer)*DreamTaq Green PCR Master mix - contains dNTPs – dilute to 2 mM each in water, make single use aliquots (50 uL aliquotspolymerase, salts, and buffer with loading dye https://www.thermofisher.. 10uL of each dNTP, 460uL water)com/order/catalog/product/K1081?ICID=search-product#/K1081?ICID=search-product *Template – generally 1uL or less of a plasmid miniprep*Polymerase – use Pfu Turbo for cloning and Taq for noncloning. Use appropriate buffer.RNAse-Free water - comes in DreamTaq kit