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==PRIMERS==
*Fwd1: 5' GAT TCC CGC CCG AAT TAG C 3'
*Fwd2: 5' CCG AAT TAG CCT GGT CAC CC 3'
*Rev: 5’ ATC CGT CCT ACT CTG GCT AAG 3'
Make primers at 0.4uM (Fwd1 + Rev and Fwd2 + Rev). PCR primers separately.
==PCR PROTOCOL==
{|border="1"
!| PCR Step || Temperature || Time || Cycles || Notes
|-
|1. Initiation/Melting || 95 || 3:00 || 1 ||
|-
|2. Denaturation || 95 || 0:30 || 38 ||
|-
|3. Annealing || 60 || 0:40 || 38 ||
|-
|4. Elongation || 72 || 1:00 || 38 || steps 2-4 to be run in sequence
|-
|5. Amplification || 72 || 5:00 || 1 ||
|-
|6. Finish || 4 || infinite || n/a ||
|}
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]
*Fwd1: 5' GAT TCC CGC CCG AAT TAG C 3'
*Fwd2: 5' CCG AAT TAG CCT GGT CAC CC 3'
*Rev: 5’ ATC CGT CCT ACT CTG GCT AAG 3'
Make primers at 0.4uM (Fwd1 + Rev and Fwd2 + Rev). PCR primers separately.
==PCR PROTOCOL==
{|border="1"
!| PCR Step || Temperature || Time || Cycles || Notes
|-
|1. Initiation/Melting || 95 || 3:00 || 1 ||
|-
|2. Denaturation || 95 || 0:30 || 38 ||
|-
|3. Annealing || 60 || 0:40 || 38 ||
|-
|4. Elongation || 72 || 1:00 || 38 || steps 2-4 to be run in sequence
|-
|5. Amplification || 72 || 5:00 || 1 ||
|-
|6. Finish || 4 || infinite || n/a ||
|}
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]
