Weigh-Suckle-Weigh Milk Volume Measurement - Revision history

Reddj at 17:42, 8 April 2021

2021-04-08T17:42:32Z

← Older revision		Revision as of 17:42, 8 April 2021
Line 15:		Line 15:
	Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0		Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0

	Pups wt 22.71 ---> after 2 hour separation 22.55 ---> after ~~2 hours~~ nursing 23.20g		Pups wt 22.71 ---> after 2 hour separation 22.55 ---> after 1 hour nursing 23.20g


	Note 2:		Note 2:
	* The weight of the dam may decrease after 2-hour separation from pups, do not worry about that (despite assuming the dam should retain more milk and gain weight).		* The weight of the dam may decrease after 2-hour separation from pups, do not worry about that (despite assuming the dam should retain more milk and gain weight).

Elhabbal at 21:38, 22 December 2020

2020-12-22T21:38:15Z

← Older revision		Revision as of 21:38, 22 December 2020
Line 14:		Line 14:
	* The data collected should look something like this for the dam and pups for all time points. This is an example to highlight the 3 data points for weight measurements.		* The data collected should look something like this for the dam and pups for all time points. This is an example to highlight the 3 data points for weight measurements.
	Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0		Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0

	Pups wt 22.71 ---> after 2 hour separation 22.55 ---> after 2 hours nursing 23.20g		Pups wt 22.71 ---> after 2 hour separation 22.55 ---> after 2 hours nursing 23.20g

Elhabbal: Weigh-suckle-weigh protocol to measure milk output per dam at PND10.5 protocol created

2020-12-22T21:37:46Z

Weigh-suckle-weigh protocol to measure milk output per dam at PND10.5 protocol created

Elhabbal: Created page with "Weigh-Suckle-Weigh Protocol at PND10.5 1. This is usually done at PND10.5 (Usually denote PND0.5 as the day pups were born). 2. Weigh dam alone using the regular metric weigh..."

2020-12-22T21:34:36Z

Created page with "Weigh-Suckle-Weigh Protocol at PND10.5 1. This is usually done at PND10.5 (Usually denote PND0.5 as the day pups were born). 2. Weigh dam alone using the regular metric weigh..."

New page

Weigh-Suckle-Weigh Protocol at PND10.5

1. This is usually done at PND10.5 (Usually denote PND0.5 as the day pups were born).
2. Weigh dam alone using the regular metric weighing scale (in the procedure room) and weigh aggregate of pups by placing all pups on weighing paper using the analytical scale (in the lab).
3. Keep the dam in the original cage with food and water bottle ad-lib. Place pups in a new cage without food or water, but keep the normal cage enrichment (the bedding pouch, tear it open, and put it on top of pups as a blanket).
4. Put the pup cage on a heating pad (set heat to “high”) so they will remain warm for the separation period. Put ¾ of the cage on the pad and leave a ¼ on the silver bench so pups can choose to move around if they get too warm. I usually place the dam’s cage next to the pups with the similar ¾ ¼ alignment on the heating pad and bench, respectively.
5. Keep the pups and dam separated for two hours.
6. After two hours of separation, weigh the pup aggregate weight on the analytical scale and weigh the dam using the metric weighing scale.
7. Recombine the pups and dam in the original cage.
8. After one hour of nursing, weigh the dam again using the metric weighing scale then weigh the aggregate of pups using the analytical scale.
Notes: The data collected should look something like this for the dams and pups at each time point.
Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0
Pups wt 22.71 ---> after 2 hour separation 22.55 --->after 2 hours nursing 23.20g

Notes: The weight of the dam may decrease after 2-hour separation from pups, do not worry about that (despite assuming the dam should retain more milk and gain weight).

← Older revision		Revision as of 21:37, 22 December 2020
Line 1:		Line 1:
	Weigh-Suckle-Weigh Protocol at PND10.5		Weigh-Suckle-Weigh Protocol at PND10.5

	1. This is usually done at PND10.5 (Usually denote PND0.5 as the day pups were born).		* This is usually done at PND10.5 (Usually denote PND0.5 as the day pups were born).
	2. Weigh dam alone using the regular metric weighing scale (in the procedure room) and weigh aggregate of pups by placing all pups on weighing paper using the analytical scale (in the lab).		* Weigh dam alone using the regular metric weighing scale (in the procedure room) and weigh aggregate of pups by placing all pups on weighing paper using the analytical scale (in the lab).
	3. Keep the dam in the original cage with food and water bottle ad-lib. Place pups in a new cage without food or water, but keep the normal cage enrichment (the bedding pouch, tear it open, and put it on top of pups as a blanket).		* Keep the dam in the original cage with food and water bottle ad-lib. Place pups in a new cage without food or water, but keep the normal cage enrichment (the bedding pouch, tear it open, and put it on top of pups as a blanket).
	4. Put the pup cage on a heating pad (set heat to “high”) so they will remain warm for the separation period. Put ¾ of the cage on the pad and leave a ¼ on the silver bench so pups can choose to move around if they get too warm. I usually place the dam’s cage next to the pups with the similar ¾ ¼ alignment on the heating pad and bench, respectively.		* Put the pup cage on a heating pad (set heat to “high”) so they will remain warm for the separation period. Put ¾ of the cage on the pad and leave a ¼ on the silver bench so pups can choose to move around if they get too warm. I usually place the dam’s cage next to the pups with the similar ¾ ¼ alignment on the heating pad and bench, respectively.
	5. Keep the pups and dam separated for two hours.		* Keep the pups and dam separated for two hours.
	6. After two hours of separation, weigh the pup aggregate weight on the analytical scale and weigh the dam using the metric weighing scale.		* After two hours of separation, weigh the pup aggregate weight on the analytical scale and weigh the dam using the metric weighing scale.
	7. Recombine the pups and dam in the original cage.		* Recombine the pups and dam in the original cage.
	8. After one hour of nursing, weigh the dam again using the metric weighing scale then weigh the aggregate of pups using the analytical scale.		* After one hour of nursing, weigh the dam again using the metric weighing scale then weigh the aggregate of pups using the analytical scale.
	~~Notes~~: The data collected should look something like this for the ~~dams~~ and pups ~~at each~~ time ~~point~~.

			Note 1:
			* The data collected should look something like this for the dam and pups for all time points. This is an example to highlight the 3 data points for weight measurements.
	Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0		Dam wt 26.9g ---> after 2 hour separation 25.7g ---> after 1 hour nursing 25.0
	Pups wt 22.71 ---> after 2 hour separation 22.55 --->after 2 hours nursing 23.20g		Pups wt 22.71 ---> after 2 hour separation 22.55 ---> after 2 hours nursing 23.20g


	~~Notes~~: The weight of the dam may decrease after 2-hour separation from pups, do not worry about that (despite assuming the dam should retain more milk and gain weight).		Note 2:
			* The weight of the dam may decrease after 2-hour separation from pups, do not worry about that (despite assuming the dam should retain more milk and gain weight).