TaqMan SNP Assay - Revision history

Lmcallan at 16:39, 16 November 2015

2015-11-16T16:39:59Z

2015-11-16T16:39:40Z

← Older revision		Revision as of 16:39, 16 November 2015
Line 16:		Line 16:
	# Dilute gDNA samples to 20ng/ul approx.		# Dilute gDNA samples to 20ng/ul approx.
	# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.		# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
	# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X)).		# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).
	# Add Reaction Mix (5.25 ul) added to each well (Table 1.)		# Add Reaction Mix (5.25 ul) added to each well (Table 1.)
	# Diluted gDNA samples each added to a single well (Table 1.)		# Diluted gDNA samples each added to a single well (Table 1.)

2015-11-16T16:38:48Z

← Older revision		Revision as of 16:38, 16 November 2015
Line 20:		Line 20:
	# Diluted gDNA samples each added to a single well (Table 1.)		# Diluted gDNA samples each added to a single well (Table 1.)

	'''Table 1. ~~Reaction Mix for full~~ 96 well plate'''
			'''Table 1. Full 96 well plate'''
	{\| class="wikitable"		{\| class="wikitable"
	\|-		\|-

2015-11-16T16:38:21Z

← Older revision		Revision as of 16:38, 16 November 2015
Line 14:		Line 14:

	== Protocol ==		== Protocol ==
	# Dilute gDNA samples to ng/ul.		# Dilute gDNA samples to 20ng/ul approx.
	# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.		# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
	# Make up Reaction Master mix (~~see table below~~).		# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X)).
	# Add Reaction Mix (5.25 ul) added to each well (~~see~~ Table 2.)		# Add Reaction Mix (5.25 ul) added to each well (Table 1.)
	# Diluted gDNA samples each added to a single well (~~see~~ Table 2.)		# Diluted gDNA samples each added to a single well (Table 1.)

	'''Table 1. Reaction Mix for full 96 well plate'''		'''Table 1. Reaction Mix for full 96 well plate'''
Line 35:		Line 35:
	\|26.4 ul		\|26.4 ul
	\|-		\|-
	!		\|gDNA Sample (20ng/ul)
	~~!5.25 ul~~		\|4.50
	~~!554.4 ul~~		\|501.6
	\|}




	~~'''Table 2. for full 96 well plate'''~~
	~~{\| class="wikitable"~~
	\|-
	~~! Component~~
	~~! 1X volume~~
	~~! 96X Volume~~ (~~+ 10% excess~~)
	\|-
	~~\|Reaction Master Mix~~
	~~\|5.25 ul~~
	~~\|554.4 ul~~
	\|-
	~~\|gDNA samples~~
	\|4.~~75 ul~~
	\|501.6 ul
	\|-		\|-
	!		!

2015-11-16T16:35:19Z

← Older revision		Revision as of 16:35, 16 November 2015
Line 17:		Line 17:
	# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.		# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
	# Make up Reaction Master mix (see table below).		# Make up Reaction Master mix (see table below).
			# Add Reaction Mix (5.25 ul) added to each well (see Table 2.)
			# Diluted gDNA samples each added to a single well (see Table 2.)

	'''Table 1. Reaction Mix for full 96 well plate'''		'''Table 1. Reaction Mix for full 96 well plate'''
Line 38:		Line 39:
	!554.4 ul		!554.4 ul
	\|}		\|}



	~~# Reaction Mix (5.25 ul) added to each well (see Table 2.)~~
	~~# Diluted gDNA samples each added to a single well (see Table 2.)~~

2015-11-16T16:34:34Z

Protocol

← Older revision		Revision as of 16:34, 16 November 2015
Line 38:		Line 38:
	!554.4 ul		!554.4 ul
	\|}		\|}



Line 44:		Line 43:
	# Reaction Mix (5.25 ul) added to each well (see Table 2.)		# Reaction Mix (5.25 ul) added to each well (see Table 2.)
	# Diluted gDNA samples each added to a single well (see Table 2.)		# Diluted gDNA samples each added to a single well (see Table 2.)

2015-11-16T16:29:58Z

← Older revision		Revision as of 16:29, 16 November 2015
Line 38:		Line 38:
	!554.4 ul		!554.4 ul
	\|}		\|}




	# Reaction Mix (5.25 ul) added to each well (see Table 2.)		# Reaction Mix (5.25 ul) added to each well (see Table 2.)
	# Diluted gDNA samples each added to a single well (see Table 2.)		# Diluted gDNA samples each added to a single well (see Table 2.)





	'''Table 2. for full 96 well plate'''		'''Table 2. for full 96 well plate'''

2015-11-16T16:29:27Z

← Older revision		Revision as of 16:29, 16 November 2015
Line 37:		Line 37:
	!5.25 ul		!5.25 ul
	!554.4 ul		!554.4 ul
			\|}

	# Reaction Mix (5.25 ul) added to each well (see Table 2.)		# Reaction Mix (5.25 ul) added to each well (see Table 2.)
Line 59:		Line 60:
	!10 ul		!10 ul
	!1056 ul		!1056 ul
			\|}

2015-11-16T16:28:26Z

@@ Line 14: / Line 14: @@
 == Protocol ==
-# Dilute gDNA samples to 1-5 ng/ul.
+# Dilute gDNA samples to  ng/ul.
 # Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
 # Make up Reaction Master mix (see table below).
-Master Mix for full 96 well plate
+'''Table 1. Reaction Mix for full 96 well plate'''
 {| class="wikitable"
 |-
@@ Line 37: / Line 37: @@
 !5.25 ul
 !554.4 ul

2015-11-16T16:20:09Z

← Older revision		Revision as of 16:20, 16 November 2015
Line 17:		Line 17:
	# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.		# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
	# Make up Reaction Master mix (see table below).		# Make up Reaction Master mix (see table below).


	Master Mix for full 96 well plate		Master Mix for full 96 well plate