https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Talk%3AFugene_Transfection_of_C2C12_CellsTalk:Fugene Transfection of C2C12 Cells - Revision history2026-04-20T00:55:53ZRevision history for this page on the wikiMediaWiki 1.45.1https://bridgeslab.sph.umich.edu/protocols/index.php?title=Talk:Fugene_Transfection_of_C2C12_Cells&diff=745&oldid=prevDavebrid: ethan provided his protocol2013-02-22T16:45:43Z<p>ethan provided his protocol</p>
<p><b>New page</b></p><div>from ethan s<br />
<br />
rough protocol of C2C12 transfection:<br />
<br />
grow cells in 12-well plate to ~70% confluency in 20% Cos FBS no PSG<br />
per well, add 5uL lipofectamine to 50 uL Optimem and 2ug DNA to another 50 uL Optimem, sit 5 minutes separately then combine and sit for 20 minutes<br />
change C2C12 into Optimem media, add 100 uL of DNA/lipofectamine, incubate for 6 hours at 37C<br />
switch to normal media (differentiation or Cos FBS + PSG depending on cell death).</div>Davebrid