https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=TC10b_Genotyping TC10b Genotyping - Revision history 2026-05-30T03:01:58Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=TC10b_Genotyping&diff=761&oldid=prev Davebrid: wrote initial protocol 2013-07-05T14:55:00Z <p>wrote initial protocol</p> <p><b>New page</b></p><div>==Primers==<br /> * &#039;&#039;&#039;TC10b - PDFor1&#039;&#039;&#039;: GGAGTCTATGGACATGAGAG<br /> * &#039;&#039;&#039;TC10b - PD4-43&#039;&#039;&#039;: GATCATGACTAGCATGGAGTAG<br /> * &#039;&#039;&#039;TC10b - PDHomoFB&#039;&#039;&#039;: GTCTATGGACATGAGAGTTTC<br /> * &#039;&#039;&#039;TC10b - NEO3A&#039;&#039;&#039;: GCAGCGCATCGCCTTCTATC<br /> <br /> ==Genotyping Program==<br /> * 94 2min<br /> * 50 cycles of:<br /> ** 94 30s<br /> ** 56 30s<br /> ** 72 4min<br /> * 72 7min<br /> * 4 forever<br /> <br /> ==Results==<br /> <br /> WT allele ~ 1kb, KO allele ~ 500bp<br /> HET should get two bands<br /> <br /> [[ Category:Genotyping ]]<br /> [[ Category:Mouse Work]]<br /> [[ Category:PCR]]<br /> [[ Category:DNA]]<br /> [[ Category:Molecular Biology]]</div> Davebrid