Splitting Cells - Revision history

Reddj: added SOP hyperlinks

2020-01-17T19:20:42Z

added SOP hyperlinks

← Older revision		Revision as of 19:20, 17 January 2020
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	==SOP==		==SOP==
	*[[SOP-_Biosafety Cabinet\|SOP- Biosafety Cabinet]]		*[[SOP-_Biosafety Cabinet\|SOP- Biosafety Cabinet]]
	*Cryogenic Materials		*[[SOP-_Cryogenic Materials\|SOP- Cryogenic Materials]]
	*Vacuum Pumps		*[[SOP-_Vacuum Pumps\|SOP- Vacuum Pumps]]

	==Materials==		==Materials==

2020-01-17T19:19:15Z

← Older revision		Revision as of 19:19, 17 January 2020
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	==SOP==		==SOP==
	*Biosafety Cabinet		*[[SOP-_Biosafety Cabinet\|SOP- Biosafety Cabinet]]
	*Cryogenic Materials		*Cryogenic Materials
	*Vacuum Pumps		*Vacuum Pumps

2019-01-08T14:40:49Z

← Older revision		Revision as of 14:40, 8 January 2019
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			==SOP==
			*Biosafety Cabinet
			*Cryogenic Materials
			*Vacuum Pumps

	==Materials==		==Materials==
	*Media (FBS or NCS as required): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM		*Media (FBS or NCS as required): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM

2018-08-30T14:11:22Z

included instructions to make more DMEM

@@ Line 1: / Line 1: @@
 ==Materials==
 *Media (FBS or NCS as required):  Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM
 *PBS -/-
 *0.05% Trypsin

2017-06-01T17:31:25Z

← Older revision		Revision as of 17:31, 1 June 2017
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	==Protocol==		==Protocol==
	#Warm PBS and Media in water bath		#Warm PBS and Media in water bath
	# Aspirate media		# Aspirate the plate media
	#Wash cells once with 10 mL (per 10 cm dish) PBS -/- then aspirate the PBS		#Wash cells once with 10 mL (per 10 cm dish) PBS -/- then aspirate the PBS
	#Add 1 mL trypsin and allow to sit in the hood for 2-5 min		#Add 1 mL trypsin and allow to sit in the hood for 2-5 min

2017-06-01T17:28:28Z

Protocol: added the aspiration processes and changed wash cells twice to "once"

← Older revision		Revision as of 17:28, 1 June 2017
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	==Protocol==		==Protocol==
	#Warm PBS and Media in water bath		#Warm PBS and Media in water bath
	#Wash cells ~~twice~~ with 10 mL (per 10 cm dish) PBS -/-		# Aspirate media
	#Add 1 mL trypsin and sit in the hood for 2-5 min		#Wash cells once with 10 mL (per 10 cm dish) PBS -/- then aspirate the PBS
			#Add 1 mL trypsin and allow to sit in the hood for 2-5 min
	#Add 10 mL media to each new dish		#Add 10 mL media to each new dish
	#Check cells for trypsinization, and if necessary tap the cells		#Check cells for trypsinization, and if necessary tap the cells

2014-02-10T16:58:36Z

added temperature

← Older revision		Revision as of 16:58, 10 February 2014
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	#Add 9 mL media to trypsinized cells		#Add 9 mL media to trypsinized cells
	#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)		#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)
	#Replace plates in the incubator		#Replace plates in the 37C incubator

	==Cell Specific Notes==		==Cell Specific Notes==

2013-09-16T21:15:21Z

changed serum names

← Older revision		Revision as of 21:15, 16 September 2013
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	==Materials==		==Materials==
	*Media (~~L1-~~FBS ~~for 3T3-L1, COS-FBS for others~~): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM		*Media (FBS or NCS as required): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM
	*PBS -/-		*PBS -/-
	*0.05% Trypsin		*0.05% Trypsin

2009-10-26T17:29:36Z

Protocol

← Older revision		Revision as of 17:29, 26 October 2009
Line 7:		Line 7:
	#Warm PBS and Media in water bath		#Warm PBS and Media in water bath
	#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-		#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-
	#Add 1 mL trypsin and sit in the hood		#Add 1 mL trypsin and sit in the hood for 2-5 min
	#Add 10 mL media to each new dish		#Add 10 mL media to each new dish
	#Check cells for trypsinization, and if necessary tap the cells		#Check cells for trypsinization, and if necessary tap the cells
Line 13:		Line 13:
	#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)		#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)
	#Replace plates in the incubator		#Replace plates in the incubator

	==Cell Specific Notes==		==Cell Specific Notes==
	*3T3-L1 fibroblasts have special considerations regarding confluence. See [[Differentiation of 3T3-L1 Cells]]		*3T3-L1 fibroblasts have special considerations regarding confluence. See [[Differentiation of 3T3-L1 Cells]]

2009-07-31T15:27:08Z

Cell Specific Notes

← Older revision		Revision as of 15:27, 31 July 2009
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	*3T3-L1 fibroblasts have special considerations regarding confluence. See [[Differentiation of 3T3-L1 Cells]]		*3T3-L1 fibroblasts have special considerations regarding confluence. See [[Differentiation of 3T3-L1 Cells]]
	*RAW 264.7 cells are scraped, not trypsinized. See [[Culturing RAW 264.7 Cells]]		*RAW 264.7 cells are scraped, not trypsinized. See [[Culturing RAW 264.7 Cells]]
			*S2 cells are grown at 28C without extra CO2. See [[Culturing S2 Cells]]
	[[ Category:Cell Culture ]]		[[ Category:Cell Culture ]]