Rapamycin Injections - Revision history

Reddj: added SOPs

2020-06-08T17:07:52Z

added SOPs

← Older revision		Revision as of 17:07, 8 June 2020
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			==SOP==
			*[[Safety and Animal Training]]
			*[[SOP - Needle Capping]]
			*[[SOP - Rapamycin]]

	==Materials==		==Materials==
	*Tween 80 - Sigma P1754		*Tween 80 - Sigma P1754

Mpeloqu1 at 15:03, 19 February 2014

2014-02-19T15:03:00Z

← Older revision		Revision as of 15:03, 19 February 2014
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	*Weigh out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween		*Weigh out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
	*Dissolve rapamycin at 20 mg/mL (0.0219 M) in ethanol (prepare as a 1 mL aliquot and store at -20 C).		*Dissolve rapamycin at 20 mg/mL (0.0219 M) in ethanol (prepare as a 1 mL aliquot and store at -20 C).
	*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (109 uM; use this for 1 week).		*Add 5 uL of 20 mg/mL RAPA in 1 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (109 uM; use this for 1 week).
	*IP inject 10 uL/g body weight (1 mg/kg)		*IP inject 10 uL/g body weight (1 mg/kg)

Erinstephenson at 17:35, 17 February 2014

2014-02-17T17:35:07Z

← Older revision		Revision as of 17:35, 17 February 2014
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	==Preparation==		==Preparation==
	*~~Weight~~ out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween		*Weigh out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
	*Dissolve rapamycin at 20 mg/mL in ~~water~~.		*Dissolve rapamycin at 20 mg/mL (0.0219 M) in ethanol (prepare as a 1 mL aliquot and store at -20 C).
	*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).		*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (109 uM; use this for 1 week).
	*~~Inject~~ 10 uL/g body weight		*IP inject 10 uL/g body weight (1 mg/kg)

	==Alternate Rapamycin Injection Regimen==		==Alternate Rapamycin Injection Regimen==

Erinstephenson at 17:24, 17 February 2014

2014-02-17T17:24:01Z

← Older revision		Revision as of 17:24, 17 February 2014
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	<blockquote>		<blockquote>
	rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally		rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally
	once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a		once daily with either 1 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a
	concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.		concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.
	</blockquote>		</blockquote>

Davebrid: updated vehicle information

2012-11-05T16:31:05Z

updated vehicle information

← Older revision		Revision as of 16:31, 5 November 2012
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	==Materials==		==Materials==
	*Tween 80 - Sigma P1754		*Tween 80 - Sigma P1754
	*PEG - Sigma P3265		*PEG 400 Liquid - Sigma P3265

	==Preparation==		==Preparation==
	*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.		*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
	*Dissolve rapamycin at 20 mg/mL in water.		*Dissolve rapamycin at 20 mg/mL in water.
	*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).		*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).

Davebrid: added rapamycin treatment from Lamming et al

2012-09-03T17:35:26Z

added rapamycin treatment from Lamming et al

← Older revision		Revision as of 17:35, 3 September 2012
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	*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).		*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
	*Inject 10 uL/g body weight		*Inject 10 uL/g body weight

			==Alternate Rapamycin Injection Regimen==
			* Phenotypes seem to be different with rapamycin as treated by Lamming et al.
			* This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135
			<blockquote>
			rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally
			once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a
			concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.
			</blockquote>

Davebrid: added dosage volume

2011-06-23T18:02:37Z

added dosage volume

← Older revision		Revision as of 18:02, 23 June 2011
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	*Dissolve rapamycin at 20 mg/mL in water.		*Dissolve rapamycin at 20 mg/mL in water.
	*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).		*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
			*Inject 10 uL/g body weight

Nicwhite: /* Preparation */

2009-07-28T15:10:02Z

Preparation

← Older revision		Revision as of 15:10, 28 July 2009
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	==Preparation==		==Preparation==
	*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip		*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.
	*Dissolve rapamycin at 20 mg/mL		*Dissolve rapamycin at 20 mg/mL in water.
			*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).

Davebrid: initial page

2009-07-27T13:51:38Z

initial page

New page

==Materials==
*Tween 80 - Sigma P1754
*PEG - Sigma P3265

==Preparation==
*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip
*Dissolve rapamycin at 20 mg/mL