https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Rab5_FRET_Assay 2026-06-02T00:36:39Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=Rab5_FRET_Assay&diff=519&oldid=prev 2010-09-16T14:18:46Z

<p>wrote initial protocol</p> <p><b>New page</b></p><div>[[Category:FRET]]<br /> [[Category:Swanson]]<br /> [[Category:CLCI]]<br /> <br /> ==Materials==<br /> *mCitrine-Rab5BD (from EEA1/NT)<br /> *mCFP-Rab5A<br /> *Acid etched Coverslips (see [[Acid Etching Coverslips]])<br /> *Cells of interest<br /> <br /> ==Protocol==<br /> *Plate out ~150 000 cells per well in a 6 well dish with an acid etched coverslip<br /> *The next morning transfect cells with [[Fugene Transfection of 293T/COS Cells|Fugene]], [[Lipofectamine Plasmid Transfection|Lipofectamine]] or other protocol<br /> *That evening replace with fresh media (and serum starve if doing insulin/growth factor stimulation</div>

Davebrid