RT-PCR primer design for ChIP - Revision history

Davebrid: added categories

2016-08-21T14:24:23Z

added categories

← Older revision		Revision as of 14:24, 21 August 2016
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	# Like always you should print off this page and keep it in your records, indicating the primer pair you ordered, the NM# (if one is associated) and the sequences and locations of the forward and reverse primers. Remember to record these primers in the primer database as well.		# Like always you should print off this page and keep it in your records, indicating the primer pair you ordered, the NM# (if one is associated) and the sequences and locations of the forward and reverse primers. Remember to record these primers in the primer database as well.
	# Order primers from IDT [https://www.idtdna.com/site]. It is important to indicate on the primer database and in the name on the label that these are ChIP primers so as not to be confused with normal qPCR primers.		# Order primers from IDT [https://www.idtdna.com/site]. It is important to indicate on the primer database and in the name on the label that these are ChIP primers so as not to be confused with normal qPCR primers.

			[[ Category: Immunoprecipitation‏‎ ]]
			[[ Category: Transcription ]]
			[[ Category: PCR ]]

Iharvey at 17:19, 25 January 2016

2016-01-25T17:19:22Z

← Older revision		Revision as of 17:19, 25 January 2016
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	# Locate the potential gene regions you believe your protein is bound to (for ~~glucocorticoid-induced~~ ChIP-seq peaks refer to [[Locating ChIP-seq peaks from ENCODE]]. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.		# Locate the potential gene regions you believe your protein is bound to (for ChIP-seq peaks refer to [[Locating ChIP-seq peaks from ENCODE]]. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
	# The genetic region entered for primer search should be around 400 bp.		# The genetic region entered for primer search should be around 400 bp.
	# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]		# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]

Iharvey at 17:18, 25 January 2016

2016-01-25T17:18:19Z

← Older revision		Revision as of 17:18, 25 January 2016
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	# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to [[Locating ChIP-seq ~~Peaks~~ from ENCODE]]. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.		# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to [[Locating ChIP-seq peaks from ENCODE]]. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
	# The genetic region entered for primer search should be around 400 bp.		# The genetic region entered for primer search should be around 400 bp.
	# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]		# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]

Iharvey at 17:17, 25 January 2016

2016-01-25T17:17:24Z

← Older revision		Revision as of 17:17, 25 January 2016
Line 1:		Line 1:
	# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks ~~protocol)~~. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.		# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to [[Locating ChIP-seq Peaks from ENCODE]]. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
	# The genetic region entered for primer search should be around 400 bp.		# The genetic region entered for primer search should be around 400 bp.
	# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]		# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]

Iharvey: Created page with "# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks protocol). Make sure this..."

2016-01-25T16:44:53Z

Created page with "# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks protocol). Make sure this..."

New page

# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks protocol). Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
# The genetic region entered for primer search should be around 400 bp.
# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]
# Enter your sequence in the first box.
# PCR product size should be set to 70-150bp.
# Make sure to select the proper species in the “organism” section.
# Click ‘Get Primers’.
# Choose a primer pair that is towards the middle region, if available.
# Like always you should print off this page and keep it in your records, indicating the primer pair you ordered, the NM# (if one is associated) and the sequences and locations of the forward and reverse primers. Remember to record these primers in the primer database as well.
# Order primers from IDT [https://www.idtdna.com/site]. It is important to indicate on the primer database and in the name on the label that these are ChIP primers so as not to be confused with normal qPCR primers.