https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Quantification_of_Vacuolar_Colocalization 2026-06-01T22:13:25Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=Quantification_of_Vacuolar_Colocalization&diff=482&oldid=prev 2010-06-11T13:42:31Z

<p>wrote initial page</p> <p><b>New page</b></p><div>==Software==<br /> *ImageJ - available at http://rsbweb.nih.gov/ij/<br /> <br /> ==Method==<br /> #You Need A Merged High Resolution Image<br /> #Open File in ImageJ<br /> #Go To Image -&gt; Color -&gt; Channels Tool<br /> #From the dropdown menu select Color then click OK<br /> #Zoom in to see the cell<br /> #Draw a Line using the Line tool. The line should start inside the cell, and cross the vacuole twice. Try to pick a representative cross section of the vacuole<br /> #Select Analyse -&gt; Plot Profile (or hit the shortcut CTRL-K)<br /> #On the popup window select copy and paste the data into an excel document<br /> #Move the slide bar on the bottom of the image to the green channel<br /> #Select Analyse -&gt; Plot Profile (or hit the shortcut CTRL-K)<br /> #On the popup window select copy and paste the data into an excel document</div>

Davebrid