https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Preparation_of_Orthovanadate_Stocks 2026-06-02T00:36:31Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=Preparation_of_Orthovanadate_Stocks&diff=1365&oldid=prev 2017-08-02T14:29:58Z

<p>changed category tag</p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:29, 2 August 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l19">Line 19:</td> <td colspan="2" class="diff-lineno">Line 19:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[ Category: Tissue Culture ]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[ Category: Tissue Culture ]]</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[ Category: Biochemicals ]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[ Category: Biochemicals ]]</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[ Category: <del style="font-weight: bold; text-decoration: none;">Buffers </del>]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[ Category: <ins style="font-weight: bold; text-decoration: none;">Buffer </ins>]]</div></td></tr> <!-- diff cache key bridgeslabproto-mw_:diff:1.41:old-1010:rev-1365:php=table --> </table>

Davebrid https://bridgeslab.sph.umich.edu/protocols/index.php?title=Preparation_of_Orthovanadate_Stocks&diff=1010&oldid=prev 2016-01-27T20:56:21Z

<p>Wrote initial page</p> <p><b>New page</b></p><div>Protocol from http://www.abcam.com/ps/pdf/protocols/Buffers%20and%20stock%20solutions.pdf<br /> <br /> <br /> This needs to be done in the fume hood<br /> <br /> # Prepare a 100 mM solution in double distilled water (1.83g/100mL).<br /> # Set pH to 9.0 with HCl, color will become yellow<br /> # Boil until colorless.<br /> # Cool to room temperature.<br /> # Set pH to 9.0 again.<br /> # Boil again until colorless.<br /> # Repeat this cycle until the solution remains at pH 9.0 after boiling and cooling.<br /> # Bring up to the initial volume with water.<br /> # Store in aliquots at -20°C in the box marked &#039;&#039;&#039;Protease Inhibitors&#039;&#039;&#039;<br /> Note: do not permit great changes in volume during boiling; put a loose lid on the container to protect from evaporation.<br /> Discard if the samples turn yellow.<br /> <br /> [[ Category: Protein Work ]]<br /> [[ Category: Tissue Culture ]]<br /> [[ Category: Biochemicals ]]<br /> [[ Category: Buffers ]]</div>

Davebrid