https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Preparation_of_Mouse_Muscle_for_CryosectioningPreparation of Mouse Muscle for Cryosectioning - Revision history2026-06-02T00:37:36ZRevision history for this page on the wikiMediaWiki 1.45.1https://bridgeslab.sph.umich.edu/protocols/index.php?title=Preparation_of_Mouse_Muscle_for_Cryosectioning&diff=2555&oldid=prevDavebrid: Wrote initial page2023-03-28T17:45:39Z<p>Wrote initial page</p>
<p><b>New page</b></p><div>== Materials ==<br />
* OCT<br />
* Methylbutane or Isopentane (the same thing)<br />
* Liquid Nitrogen or Dry ice (liquid nitrogen preferred)<br />
<br />
== Procedures ==<br />
* Place metal beaker of isopentane in container of liquid nitogen.<br />
* Label small pieces of popsicle sticks with mouse ids and add a drop of OCT<br />
* Label ~5 cm<sup>2</sup> pieces of aluminum foil with the mouse IDs<br />
* Rapidly dissect muscle and place on the drop of OCT. Drop the entire OCT/popsicle stick into the cold isopentane. They should stay together, but if they separate take it out and proceed to the next step<br />
* Using tweezers carefully remove the frozen muscle/OCT/wood and flip over the tin foil so the mouse id is on the outsize<br />
* Wrap up the tin foil loosely just to hold the tissue and place back in liquid nitrogen. When complete, take the tin foil out and place in a box and store at -80 until ready to section</div>Davebrid