Matrigel Basement Membrane Matrix - Revision history

Reddj at 15:15, 28 April 2017

2017-04-28T15:15:29Z

← Older revision		Revision as of 15:15, 28 April 2017
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	==RECONSTRUCTION AND USE==		==RECONSTRUCTION AND USE==

	*Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.		Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.


	1. Thaw on ice in the back of a 4°C refrigerator, overnight.		1. Thaw on ice in the back of a 4°C refrigerator, overnight.
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	4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.		4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.

	5. Divide into 100 uL aliquots.		5. Divide into 100 uL aliquots in 1.5mL Eppendorf tubes.

			*Pre-cool pipette tips.

			*Change tips whenever Matrigel is clogging the tip and/or causing the pipette to aspirate inaccurately.

Reddj at 14:18, 28 April 2017

2017-04-28T14:18:38Z

← Older revision		Revision as of 14:18, 28 April 2017
Line 8:		Line 8:
	*Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.		*Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.

	1. Thaw on ice in the back of a 4°C refrigerator, overnight.		1. Thaw on ice in the back of a 4°C refrigerator, overnight.
	2. Once thawed, swirl to ensure even distribution.
	3. Keep on ice at all times-incuding while aliquoting.		2. Once thawed, swirl to ensure even distribution.
	4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.
	5. ~~Make~~ 100 uL aliquots.		3. Keep on ice at all times-incuding while aliquoting.

			4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.

			5. Divide into 100 uL aliquots.

Reddj: Created page with "==STORAGE== Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN. ==RECONSTRUCTION AND USE== *Color variation may occur betwee..."

2017-04-28T14:15:33Z

Created page with "==STORAGE== Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN. ==RECONSTRUCTION AND USE== *Color variation may occur betwee..."

New page

==STORAGE==

Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN.

==RECONSTRUCTION AND USE==

*Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.

1. Thaw on ice in the back of a 4°C refrigerator, overnight.
2. Once thawed, swirl to ensure even distribution.
3. Keep on ice at all times-incuding while aliquoting.
4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.
5. Make 100 uL aliquots.