Maintenance of Fly Stocks - Revision history

Davebrid: added food cost

2013-01-11T14:58:20Z

added food cost

← Older revision		Revision as of 14:58, 11 January 2013
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	Generally flies are flipped into a new vial every generation. The larvae placed into the old vial will repopulate the new vial. Keep one old vial and one new vial banded together.		Generally flies are flipped into a new vial every generation. The larvae placed into the old vial will repopulate the new vial. Keep one old vial and one new vial banded together.
	A fly generation is 10 days. To see if new larvae are being born, look for tiny black specks in the media after about 10 days. If need be use the microscope to look. Since generations are too fast, there is no reason to keep track of generations. Try to flip >20 (10 M, 10F) flies into a new vial to avoid genetic drift. No need to separate homozygotes from heterozygotes.		A fly generation is 10 days. To see if new larvae are being born, look for tiny black specks in the media after about 10 days. If need be use the microscope to look. Since generations are too fast, there is no reason to keep track of generations. Try to flip >20 (10 M, 10F) flies into a new vial to avoid genetic drift. No need to separate homozygotes from heterozygotes.

			==Food==
			* Buying food already in tubes costs $20 for 100 tubes.

	==Flipping Flies==		==Flipping Flies==

Davebrid: added note about expanding colonies

2012-11-21T19:29:37Z

added note about expanding colonies

← Older revision		Revision as of 19:29, 21 November 2012
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	==Theoretical Considerations==		==Theoretical Considerations==
	Generally flies are flipped into a new vial every generation. The larvae placed into the old vial will repopulate the new vial. Keep one old vial and one new vial banded together.		Generally flies are flipped into a new vial every generation. The larvae placed into the old vial will repopulate the new vial. Keep one old vial and one new vial banded together.
	A fly generation is 10 days. To see if new larvae are being born, look for tiny black specks in the media after about 10 days. If need be use the microscope to look. Since generations are too fast, there is no reason to keep track of generations. Try to flip >20 flies into a new vial to avoid genetic drift. No need to separate homozygotes from heterozygotes.		A fly generation is 10 days. To see if new larvae are being born, look for tiny black specks in the media after about 10 days. If need be use the microscope to look. Since generations are too fast, there is no reason to keep track of generations. Try to flip >20 (10 M, 10F) flies into a new vial to avoid genetic drift. No need to separate homozygotes from heterozygotes.

	==Flipping Flies==		==Flipping Flies==
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	If you dont get all the flies into the new vial its ok, but you should get >20 flies into the new one.		If you dont get all the flies into the new vial its ok, but you should get >20 flies into the new one.

			==Expanding Colonies==
			# Grab a conical flask.
			# Flip about 40F and 10-40M flies into the conical.

Davebrid: wrote initial protocol

2012-11-16T20:54:54Z

wrote initial protocol

New page

[[ Category: Drosophila ]]
[[ Category: Genotyping ]]
[[ Category: Maintenance ]]

==Theoretical Considerations==
Generally flies are flipped into a new vial every generation. The larvae placed into the old vial will repopulate the new vial. Keep one old vial and one new vial banded together.
A fly generation is 10 days. To see if new larvae are being born, look for tiny black specks in the media after about 10 days. If need be use the microscope to look. Since generations are too fast, there is no reason to keep track of generations. Try to flip >20 flies into a new vial to avoid genetic drift. No need to separate homozygotes from heterozygotes.

==Flipping Flies==
# Grab a new vial and take out the cotton ball
# Gently tap the older vial on the counter (on a mousepad) to drop all the flies down to the media
# Take out the old vial's cotton ball then flip the new vial on top of it, inverted
# Flip both vials and tap on the surface to move flies to the new vial.

If you dont get all the flies into the new vial its ok, but you should get >20 flies into the new one.