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Davebrid: /* Lab Safety */

2016-08-15T12:10:53Z

Lab Safety

← Older revision		Revision as of 12:10, 15 August 2016
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	==Lab Safety==		==Lab Safety==
	* For notes about required training courses see [[ Safety and Animal Training ]]		* For notes about required training courses see [[ Safety and Animal Training ]]
	* For lab-specific standard operating procedures see [[ :Category:SOP]]		* For lab-specific standard operating procedures see [[:Category: SOP]]

	==Common Protocols==		==Common Protocols==

Davebrid: Added SOP's to front page

2016-08-15T12:10:21Z

Added SOP's to front page

← Older revision		Revision as of 12:10, 15 August 2016
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	See [[Special:Categories]] for as listing of protocols by category.		See [[Special:Categories]] for as listing of protocols by category.

	==Cell and Tissue Culture==		==Lab Safety==
			* For notes about required training courses see [[ Safety and Animal Training ]]
			* For lab-specific standard operating procedures see [[ :Category:SOP]]

			==Common Protocols==

			===Cell and Tissue Culture===
	*[[Splitting Cells]]		*[[Splitting Cells]]
	*[[Generating DMSO Stocks for Cell Culture]]		*[[Generating DMSO Stocks for Cell Culture]]
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	*[[Inositol Labeling and Lipid Extraction]]		*[[Inositol Labeling and Lipid Extraction]]

	==Cloning and Molecular Biology==		===Cloning and Molecular Biology===
	*[[Transformation of Bacteria]]		*[[Transformation of Bacteria]]
	*[[Mutagenesis]]		*[[Mutagenesis]]
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	*[[Designing and Generating CRISPR-Cas Mutants]]		*[[Designing and Generating CRISPR-Cas Mutants]]

	==Cell Biology==		===Cell Biology===
	*[[Immunofluoresence]]		*[[Immunofluoresence]]
	**[[Colocalization]]		**[[Colocalization]]
	*[[FM4-64 Labeling of Yeast Cells]]		*[[FM4-64 Labeling of Yeast Cells]]

	==Protein Analysis==		===Protein Analysis===
	*[[Preparing a SDS-PAGE Gel]]		*[[Preparing a SDS-PAGE Gel]]
	*[[Western Blotting]]		*[[Western Blotting]]
	*[[Surface Plasmon Resonance - Protein Lipid Interactions]]		*[[Surface Plasmon Resonance - Protein Lipid Interactions]]
	===Protein Quantification===		====Protein Quantification====
	*[[Bradford Assay]]		*[[Bradford Assay]]
	*[[Quantification by Absorbance at 280nm]]		*[[Quantification by Absorbance at 280nm]]
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	*[[Using ImageJ to Quantify Bands]]		*[[Using ImageJ to Quantify Bands]]

	===Protein Purification===		====Protein Purification====
	*[[French Press]]		*[[French Press]]
	*[[Purification of GST Fusion Proteins]]		*[[Purification of GST Fusion Proteins]]
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	*[[Triglyceride Assay from Drosophila (German Method)]]		*[[Triglyceride Assay from Drosophila (German Method)]]

	==Transcriptional Analysis==		===Transcriptional Analysis===
	*[[Real Time PCR From Cell Culture]]		*[[Real Time PCR From Cell Culture]]
	*[[Harvesting RNA from Cells grown in monolayer]]		*[[Harvesting RNA from Cells grown in monolayer]]
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	*[[Using Bioconductor To Analyse Microarray Data]]		*[[Using Bioconductor To Analyse Microarray Data]]

	==Mouse/Fly Protocols==		===Mouse/Fly Protocols===
	*[[Maternal Particulate Exposure Breeding]]		*[[Maternal Particulate Exposure Breeding]]

	==Fly Stocks==		===Fly Stocks===
	*[[Maintenance of 18C Fly Stocks]]		*[[Maintenance of 18C Fly Stocks]]
	*[[Fly Food Protocol]]		*[[Fly Food Protocol]]
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	*[[Monitoring Food Intake]]		*[[Monitoring Food Intake]]

	==Tissue Preparation==		===Tissue Preparation===
	*[[Harvesting Mouse Tissue]]		*[[Harvesting Mouse Tissue]]
	*[[Preparation of Protein Lysates from Mouse Tissues]]		*[[Preparation of Protein Lysates from Mouse Tissues]]
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	*[[Perfusion of Mouse]]		*[[Perfusion of Mouse]]

	==Media and Buffers==		===Media and Buffers===
	===Yeast Media===		====Yeast Media====
	*[[YPD Media and Agar]]		*[[YPD Media and Agar]]
	*[[Yeast Selective Media and Agar]]		*[[Yeast Selective Media and Agar]]
	===Buffers===		====Buffers====
	====Lysis Buffers====		=====Lysis Buffers=====
	*[[KRBH Buffer]]		*[[KRBH Buffer]]
	*[[RIPA Buffer]]		*[[RIPA Buffer]]
	*[[CHAPS Lysis Buffer]]		*[[CHAPS Lysis Buffer]]
	====SDS-PAGE Buffers====		=====SDS-PAGE Buffers=====
	*[[SDS-PAGE Separating Gel Buffer]]		*[[SDS-PAGE Separating Gel Buffer]]
	*[[SDS-PAGE Stacking Gel Buffer]]		*[[SDS-PAGE Stacking Gel Buffer]]
	*[[TORC1 Kinase Buffer]]		*[[TORC1 Kinase Buffer]]
	*[[Acrylamide Solution]]		*[[Acrylamide Solution]]
	====Other Buffers====		=====Other Buffers=====
	*[[KRBH Buffer]]		*[[KRBH Buffer]]
	*[[MTORC1 Kinase Assay]]		*[[MTORC1 Kinase Assay]]

Davebrid: Changed dept website link

2016-07-24T02:23:08Z

Changed dept website link

← Older revision		Revision as of 02:23, 24 July 2016
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	Welcome to the Bridges Lab protocol site. If you would like to know more about our group, please visit the [http://bridgeslab.~~uthsc~~.edu Bridges Lab Website]. All of our experimental protocols are available through this site and are free to use/copy/modify by anyone without any restrictions. You can locate protocols by looking at this index, searching by category (see below) or searching by keyword. If you notice an error, would like to discuss these methods or would like to be able to edit these pages contact us via email.		Welcome to the Bridges Lab protocol site. If you would like to know more about our group, please visit the [http://bridgeslab.sph.umich.edu Bridges Lab Website]. All of our experimental protocols are available through this site and are free to use/copy/modify by anyone without any restrictions. You can locate protocols by looking at this index, searching by category (see below) or searching by keyword. If you notice an error, would like to discuss these methods or would like to be able to edit these pages contact us via email.

	__NOTOC__		__NOTOC__

Lmcallan: /* Genotyping */

2015-11-11T15:30:26Z

Genotyping

← Older revision		Revision as of 15:30, 11 November 2015
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	*[[Colony PCR]]		*[[Colony PCR]]
	*[[BDNF PCR]]		*[[BDNF PCR]]
			*[[TaqMan SNP Assay]]

	===Metabolic Measurements===		===Metabolic Measurements===
	*[[Glucose Tolerance Test]]		*[[Glucose Tolerance Test]]

Aragaus1 at 16:28, 2 April 2015

2015-04-02T16:28:04Z

← Older revision		Revision as of 16:28, 2 April 2015
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	==Mouse/Fly Protocols==		==Mouse/Fly Protocols==
	*Maternal Particulate Exposure Breeding		*[[Maternal Particulate Exposure Breeding]]

	==Fly Stocks==		==Fly Stocks==

Aragaus1: /* Mouse/Fly Protocols */

2015-04-02T16:27:40Z

Mouse/Fly Protocols

← Older revision		Revision as of 16:27, 2 April 2015
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	==Mouse/Fly Protocols==		==Mouse/Fly Protocols==
	*Maternal Particulate Exposure Breeding ~~Protocol~~		*Maternal Particulate Exposure Breeding

	==Fly Stocks==		==Fly Stocks==

Aragaus1: /* Mouse/Fly Protocols */

2015-04-02T16:27:27Z

Mouse/Fly Protocols

← Older revision		Revision as of 16:27, 2 April 2015
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	==Mouse/Fly Protocols==		==Mouse/Fly Protocols==
			*Maternal Particulate Exposure Breeding Protocol

	==Fly Stocks==		==Fly Stocks==
	*[[Maintenance of 18C Fly Stocks]]		*[[Maintenance of 18C Fly Stocks]]

Aragaus1: /* Tissue Preparation */

2015-03-31T18:21:51Z

Tissue Preparation

← Older revision		Revision as of 18:21, 31 March 2015
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	*[[Paraffin Embedding of Tissue Samples]]		*[[Paraffin Embedding of Tissue Samples]]
	*[[H&E Staining of Mouse Tissue]]		*[[H&E Staining of Mouse Tissue]]
			**[[Adipocyte Cell Counting with ImageJ]]
	*[[Perfusion of Mouse]]		*[[Perfusion of Mouse]]

Aragaus1: /* Tissue Preparation */

2015-03-27T17:11:00Z

Tissue Preparation

← Older revision		Revision as of 17:11, 27 March 2015
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	*[[Paraffin Embedding of Tissue Samples]]		*[[Paraffin Embedding of Tissue Samples]]
	*[[H&E Staining of Mouse Tissue]]		*[[H&E Staining of Mouse Tissue]]
			*[[Perfusion of Mouse]]

	==Media and Buffers==		==Media and Buffers==

Aragaus1: /* Tissue Preparation */

2015-03-24T19:43:34Z

Tissue Preparation

← Older revision		Revision as of 19:43, 24 March 2015
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	*[[Preparation of RNA Samples from Mouse Tissues]]		*[[Preparation of RNA Samples from Mouse Tissues]]
	*[[Paraffin Embedding of Tissue Samples]]		*[[Paraffin Embedding of Tissue Samples]]
	*[[H&E Staining of Mouse Tissue}}		*[[H&E Staining of Mouse Tissue]]

	==Media and Buffers==		==Media and Buffers==