https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=MTM1_Genotyping
MTM1 Genotyping - Revision history
2026-05-09T23:51:32Z
Revision history for this page on the wiki
MediaWiki 1.45.1
https://bridgeslab.sph.umich.edu/protocols/index.php?title=MTM1_Genotyping&diff=742&oldid=prev
Davebrid: Added categories
2013-01-21T15:17:07Z
<p>Added categories</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:17, 21 January 2013</td>
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<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[ Category:Molecular Biology]]</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153</div></td></tr>
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Davebrid
https://bridgeslab.sph.umich.edu/protocols/index.php?title=MTM1_Genotyping&diff=741&oldid=prev
Davebrid: added sample pcr result
2013-01-21T15:16:18Z
<p>added sample pcr result</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:16, 21 January 2013</td>
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Davebrid
https://bridgeslab.sph.umich.edu/protocols/index.php?title=MTM1_Genotyping&diff=739&oldid=prev
Davebrid: wrote protocol for MTM1 genotyping
2013-01-21T15:13:43Z
<p>wrote protocol for MTM1 genotyping</p>
<p><b>New page</b></p><div>See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153<br />
<br />
==Primers==<br />
<br />
*'''MTM1 Fwd''': GGC CCC ATT AAG GGA AGA GT<br />
*'''MTM1 WT Rev''': GAT CTT CTG GGA AAG GCA CA<br />
*'''MTM1 Mut Rev''': GCC AGA GGC CAC TTG TGT AG<br />
<br />
==PCR Program==<br />
<br />
* 94 for 2 min<br />
* 10 cycles of:<br />
** 94 for 20sec <br />
** 65 for 15sec with -0.5 C per cycle<br />
** 68 for 10sec <br />
* 28 cycles of:<br />
** 94 for 15sec<br />
** 60 for 15sec<br />
** 72 for 10sec<br />
* 72 for 1 min<br />
* 4 hold<br />
<br />
==Expected Results==</div>
Davebrid