https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Lipid_TransfectionLipid Transfection - Revision history2026-06-01T22:14:03ZRevision history for this page on the wikiMediaWiki 1.45.1https://bridgeslab.sph.umich.edu/protocols/index.php?title=Lipid_Transfection&diff=565&oldid=prevDavebrid: Created page with '==Materials== * Shuttle Kit from Echelon [http://www.echelon-inc.com/commerce/ccc1021-shuttle-pip-kits.htm] * I used C8 lipids and dissolved to 1 mM in PBS and frozen in small al...'2011-03-09T19:55:00Z<p>Created page with '==Materials== * Shuttle Kit from Echelon [http://www.echelon-inc.com/commerce/ccc1021-shuttle-pip-kits.htm] * I used C8 lipids and dissolved to 1 mM in PBS and frozen in small al...'</p>
<p><b>New page</b></p><div>==Materials==<br />
* Shuttle Kit from Echelon [http://www.echelon-inc.com/commerce/ccc1021-shuttle-pip-kits.htm]<br />
* I used C8 lipids and dissolved to 1 mM in PBS and frozen in small aliquots at -80. <br />
* Dissolved the appropriate carrier to 1 mM in PBS and freeze at -80 in small aliquots<br />
* For PIP use Carrier 3, for PIP2 use Carrier 2, for PIP3 use Carrier 2<br />
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==Procedure==<br />
* serum starved my cells for 3h in DMEM + PSG, using a small volume of media (~200 uL in a 12 well dish)<br />
* combined lipid and carrier 1:1 making enough to bring the final concentration on the cells to 1 uM<br />
* incubated lipid and carrier on the bench for 30 min<br />
* added mixture to cells for 30 min<br />
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[[Category: Phospholipids]]<br />
[[Category: Transfection]]</div>Davebrid