https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=KRBH_Buffer 2026-06-01T22:14:53Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=KRBH_Buffer&diff=400&oldid=prev 2009-10-14T18:27:31Z

<p>initial page</p> <p><b>New page</b></p><div>{| border=&quot;1&quot;<br /> ! || Final Concentration || per 500 mL || Stock || Location<br /> |- <br /> |HEPES pH 7.4 || 30mM || 15mL || 1M<br /> |- <br /> |Sodium bicarbonate|| 10mM || 5mL || 1M || Make fresh from powder<br /> |-<br /> |Sodium chloride || 120mM || 15mL || 4M<br /> |- <br /> |Potassium phosphate || 4mM || 2mL || 1M<br /> |- <br /> |Magnesium sulfate|| 1mM || 500uL || 1M<br /> |- <br /> |Calcium chloride || 1mM || 500uL || 1M <br /> |}<br /> *Bring up to 500 mL with ddH20<br /> *Sterile filter buffer and store at 4C<br /> [[ Category:Buffer ]]</div>

Davebrid