High Fat Diet Treatment - Revision history

Cdgleason: update diet table

2018-11-28T18:42:40Z

update diet table

@@ Line 66: / Line 66: @@
    | 44%
    | Peach
 |}

Mollyec: /* Diet Choices */

2017-05-22T19:33:02Z

Diet Choices

← Older revision		Revision as of 19:33, 22 May 2017
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	For more information about diets see [http://www.researchdiets.com/system/resources/BAhbBlsHOgZmIisyMDEyLzA0LzIwLzE0XzM5XzQwXzMxN19ESU9fU2VyaWVzLnBkZg/DIO%20Series.pdf research diets]		For more information about diets see [http://www.researchdiets.com/system/resources/BAhbBlsHOgZmIisyMDEyLzA0LzIwLzE0XzM5XzQwXzMxN19ESU9fU2VyaWVzLnBkZg/DIO%20Series.pdf research diets]
			for caloric content in future diets, use metabolizable energy, not physiological fuel value. Metabolizable energy is more accurate as it is derived from bomb calorimetry

	{\| border=1		{\| border=1

Mollyec: /* Diet Choices */

2017-05-22T19:31:50Z

Diet Choices

← Older revision		Revision as of 19:31, 22 May 2017
Line 29:		Line 29:
	\| Lab Diet		\| Lab Diet
	\| 5L0D		\| 5L0D
	\| 3.02 kcal/g		\| 2.91 kcal/g
	\| 5%		\| 5%
	\| 24%		\| 24%

Davebrid: updated caloric content of HFD

2014-04-10T10:43:38Z

updated caloric content of HFD

← Older revision		Revision as of 10:43, 10 April 2014
Line 39:		Line 39:
	\| Research Diets		\| Research Diets
	\| D12451		\| D12451
	\| 3.85 kcal/g		\| 4.73 kcal/g
	\| 45%		\| 45%
	\| 20%		\| 20%

Davebrid: added note about ordering C57BL/6J mice

2013-08-27T13:52:38Z

added note about ordering C57BL/6J mice

← Older revision		Revision as of 13:52, 27 August 2013
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	To place mice on a high fat diet, here is the general protocol:		To place mice on a high fat diet, here is the general protocol:

			For C57BL/6J mice, order so that they are 9 weeks of age on the monday of delivery. Start the diet on the Wednesday (10 days later).

	==Diet Choices==		==Diet Choices==

Davebrid: error in sucrose amount

2012-11-20T15:08:44Z

error in sucrose amount

← Older revision		Revision as of 15:08, 20 November 2012
Line 60:		Line 60:
	\| 10%		\| 10%
	\| 20%		\| 20%
	\| 7%		\| 17%
	\| 44%		\| 44%
	\| Peach		\| Peach

Davebrid: added header and categories

2012-11-20T14:53:43Z

added header and categories

← Older revision		Revision as of 14:53, 20 November 2012
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			[[ Category: Diet ]]
			[[ Category: Animal Studies ]]
			[[ Category: Glucose Homeostasis ]]
			[[ Category: Metabolism ]]
			[[ Category: Mouse Work ]]

	To place mice on a high fat diet, here is the general protocol:		To place mice on a high fat diet, here is the general protocol:

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	\|}		\|}

			==Treatment Protocol==

	# Start when mice are 10 weeks of age.		# Start when mice are 10 weeks of age.

Davebrid: filled in details about mouse diets

2012-11-20T14:51:36Z

filled in details about mouse diets

New page

To place mice on a high fat diet, here is the general protocol:

==Diet Choices==

For more information about diets see [http://www.researchdiets.com/system/resources/BAhbBlsHOgZmIisyMDEyLzA0LzIwLzE0XzM5XzQwXzMxN19ESU9fU2VyaWVzLnBkZg/DIO%20Series.pdf research diets]

{| border=1
|+ Food Souces and Notes about their Composition
|-
! Food
! Vendor
! Code
! Caloric Content
! Fat
! Protein
! Sucrose
! Starch
! Notes
|-
| Normal Chow Diet
| Lab Diet
| 5L0D
| 3.02 kcal/g
| 5%
| 24%
| 3.7%
| 32%
| Provided by ULAM
|-
| High Fat Diet
| Research Diets
| D12451
| 3.85 kcal/g
| 45%
| 20%
| 17%
| 7%
| Pink
|-
| Control Diet (High Sucrose)
| Research Diets
| D12450B
| 3.85 kcal/g
| 10%
| 20%
| 35%
| 31%
| Yellow
|-
| Control Diet (High Starch)
| Research Diets
| D12450H
| 3.85 kcal/g
| 10%
| 20%
| 7%
| 44%
| Peach
|}

# Start when mice are 10 weeks of age.
# For High Fat Diet mice place in cages 4 mice/cage as early as possible. Can us 5 mice/cage for normal chow/control diets.
# Re-cage (and ear tag mice if necessary) mice and replace food with ~400g food in the container. Record this weight.
# This food should last 2 weeks.
# Weigh mice at ~10 AM either weekly or bi-weekly and re-weigh food to determine food intake. Top up to 400g.
# Generally treatment lasts 12 weeks.