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	<id>https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&amp;feed=atom&amp;title=HNTG_Buffer</id>
	<title>HNTG Buffer - Revision history</title>
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	<updated>2026-05-30T23:21:53Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
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	<entry>
		<id>https://bridgeslab.sph.umich.edu/protocols/index.php?title=HNTG_Buffer&amp;diff=422&amp;oldid=prev</id>
		<title>Davebrid at 14:50, 4 November 2009</title>
		<link rel="alternate" type="text/html" href="https://bridgeslab.sph.umich.edu/protocols/index.php?title=HNTG_Buffer&amp;diff=422&amp;oldid=prev"/>
		<updated>2009-11-04T14:50:25Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;table style=&quot;background-color: #fff; color: #202122;&quot; data-mw=&quot;interface&quot;&gt;
				&lt;col class=&quot;diff-marker&quot; /&gt;
				&lt;col class=&quot;diff-content&quot; /&gt;
				&lt;col class=&quot;diff-marker&quot; /&gt;
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				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #202122; text-align: center;&quot;&gt;← Older revision&lt;/td&gt;
				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #202122; text-align: center;&quot;&gt;Revision as of 14:50, 4 November 2009&lt;/td&gt;
				&lt;/tr&gt;&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot; id=&quot;mw-diff-left-l8&quot;&gt;Line 8:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 8:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;| NaCl || 150 mM || 375 uL || 4M || Shelf&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;| NaCl || 150 mM || 375 uL || 4M || Shelf&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot; data-marker=&quot;−&quot;&gt;&lt;/td&gt;&lt;td style=&quot;color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;| Glycerol || &lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;210&lt;/del&gt;% || 1 mL || &lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;0.5M &lt;/del&gt;|| Bench&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot; data-marker=&quot;+&quot;&gt;&lt;/td&gt;&lt;td style=&quot;color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;| Glycerol || &lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;10&lt;/ins&gt;% || 1 mL || &lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt; &lt;/ins&gt;|| Bench&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;|-&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;| Triton X-100 || 1% || 0.5 mL || 20% || Shelf&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;| Triton X-100 || 1% || 0.5 mL || 20% || Shelf&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;

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&lt;/table&gt;</summary>
		<author><name>Davebrid</name></author>
	</entry>
	<entry>
		<id>https://bridgeslab.sph.umich.edu/protocols/index.php?title=HNTG_Buffer&amp;diff=421&amp;oldid=prev</id>
		<title>Davebrid: Created page with &#039; Category:Buffer   Category:Immunoprecipitation {| border=&quot;1&quot; ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C...&#039;</title>
		<link rel="alternate" type="text/html" href="https://bridgeslab.sph.umich.edu/protocols/index.php?title=HNTG_Buffer&amp;diff=421&amp;oldid=prev"/>
		<updated>2009-11-04T14:49:37Z</updated>

		<summary type="html">&lt;p&gt;Created page with &amp;#039;&lt;a href=&quot;/protocols/index.php/Category:Buffer&quot; title=&quot;Category:Buffer&quot;&gt;Category:Buffer &lt;/a&gt; &lt;a href=&quot;/protocols/index.php/Category:Immunoprecipitation&quot; title=&quot;Category:Immunoprecipitation&quot;&gt;Category:Immunoprecipitation&lt;/a&gt; {| border=&amp;quot;1&amp;quot; ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C...&amp;#039;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;[[ Category:Buffer ]]&lt;br /&gt;
[[ Category:Immunoprecipitation]]&lt;br /&gt;
{| border=&amp;quot;1&amp;quot;&lt;br /&gt;
! || Final Concentration || per 10 mL || Stock || Location&lt;br /&gt;
|-&lt;br /&gt;
| HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C&lt;br /&gt;
|-&lt;br /&gt;
| NaCl || 150 mM || 375 uL || 4M || Shelf&lt;br /&gt;
|-&lt;br /&gt;
| Glycerol || 210% || 1 mL || 0.5M || Bench&lt;br /&gt;
|-&lt;br /&gt;
| Triton X-100 || 1% || 0.5 mL || 20% || Shelf&lt;br /&gt;
|-&lt;br /&gt;
| Protease Inhibitor || || 1 tablet || ||&lt;br /&gt;
|}&lt;/div&gt;</summary>
		<author><name>Davebrid</name></author>
	</entry>
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