https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=H%26E_Staining 2026-05-10T02:09:38Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=H%26E_Staining&diff=1618&oldid=prev 2020-06-08T15:23:18Z

<p>added SOPs</p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:23, 8 June 2020</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">==SOP==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*[[SOP - Flammables and Combustibles]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*[[SOP- Irritants]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*[[SOP- Corrosives]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Rehydrate tissue sections ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Rehydrate tissue sections ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute washes using fresh xylene each time</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute washes using fresh xylene each time</div></td></tr> <!-- diff cache key bridgeslabproto-mw_:diff:1.41:old-878:rev-1618:php=table --> </table>

Reddj https://bridgeslab.sph.umich.edu/protocols/index.php?title=H%26E_Staining&diff=878&oldid=prev 2014-10-13T22:18:36Z

<p>Created page with &quot;== Rehydrate tissue sections == #After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute ...&quot;</p> <p><b>New page</b></p><div>== Rehydrate tissue sections ==<br /> #After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute washes using fresh xylene each time<br /> #Rehydrate the tissue sections by immersing in 100% ethanol for 3 minutes. Repeat a second time using fresh 100% ethanol<br /> #Immerse sections in 95% ethanol for 3 minutes. Repeat a second time using fresh 95% ethanol<br /> #Immerse sections in 70% ethanol for 3 minutes<br /> #Rinse sections 2 to 3 times in distilled water<br /> <br /> == Stain tissue sections with haematoxylin ==<br /> #Immerse sections in pre-prepared Mayer&#039;s haematoxylin for 5 minutes <br /> #Rinse under running tap water (slides still in box) until water is clear. This will take about 5 minutes<br /> <br /> == Stain tissue sections with eosin, dehydrate and coverslip ==<br /> #Immerse sections in 1% eosin from 15 seconds to 1 minute<br /> #Rinse 3-4 times under running tap water<br /> #Immerse in 70% ethanol for 2 minutes<br /> #Immerse in in 95% ethanol for 2 minutes. Repeat a second time using fresh 95% ethanol<br /> #Immerse in in 100% ethanol for 2 minutes. Repeat twice more using fresh 100% ethanol<br /> #Immerse in xylene for 2 minutes. Repeat twice more using fresh xylene. Check staining under the microscope<br /> #Coverslip using Permount or other xylene-based mounting medium</div>

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