Glucose Tolerance Test - Revision history

Davebrid: added category

2025-02-10T16:42:48Z

added category

← Older revision		Revision as of 16:42, 10 February 2025
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	#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein		#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein

			[[Category:Animal Work]]
	[[Category:Mouse Work]]		[[Category:Mouse Work]]
	[[Category:Glucose Homeostasis]]		[[Category:Glucose Homeostasis]]
	[[Category:Injections]]		[[Category:Injections]]

Davebrid: Added details about ipGTT

2023-03-28T17:37:49Z

Added details about ipGTT

← Older revision		Revision as of 17:37, 28 March 2023
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	#Remove food from mice for about 6h. Add do not feed tag to cages.		#Remove food from mice for about 6h. Add do not feed tag to cages.
	#Bring mice to procedure room. Put water bottle on cage.		#Bring mice to procedure room. Put water bottle on cage.
	#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized ~~capilary~~ about 2/3 full with blood.		#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood.
	#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).		#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
	#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.		#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test.
	##Immobilize mouse and restrain tail with one hand		##Immobilize mouse and restrain tail with one hand
	##Aim needle between the midline and the hip bone		##'''For ipGTT''' Aim needle between the midline and the hip bone
	##Insert syringe (do not inject) into cavity		##'''For oGTT''' Gently inert gavage needle into mouth
	##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over		##Insert syringe (do not inject) into cavity/mouth
			##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
	##Eject syringe.		##Eject syringe.
	#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein		#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein

	[[Category:Mouse Work]]		[[Category:Mouse Work]]
	[[Category:Glucose Homeostasis]]		[[Category:Glucose Homeostasis]]
	[[Category:Injections]]		[[Category:Injections]]

Davebrid: /* Materials (Bring to Procedure Room) */

2023-03-28T17:35:40Z

Materials (Bring to Procedure Room)

← Older revision		Revision as of 17:35, 28 March 2023
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	==Materials (Bring ~~Downstairs~~)==		==Materials (Bring to Procedure Room)==
	*Glucometer - AccuChek Advantage		*Glucometer - AccuChek Advantage
	*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy.		*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy.
Line 11:		Line 11:
	*Syringes		*Syringes
	*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.		*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
			*If doing an oral glucose tolerance test need a gavage needle
	*Timer		*Timer

Reddj: added SOP

2020-06-08T15:05:17Z

added SOP

← Older revision		Revision as of 15:05, 8 June 2020
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			==SOP==
			*[[SOP - Needle Capping]]
			*[[Safety and Animal Training]]


	==Materials (Bring Downstairs)==		==Materials (Bring Downstairs)==
	*Glucometer - AccuChek Advantage		*Glucometer - AccuChek Advantage

Irith at 16:19, 24 August 2011

2011-08-24T16:19:56Z

← Older revision		Revision as of 16:19, 24 August 2011
Line 1:		Line 1:
	==Materials (Bring Downstairs)==		==Materials (Bring Downstairs)==
	*Glucometer - AccuChek Advantage		*Glucometer - AccuChek Advantage
	*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]		*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy.
	*Scale		*Scale
	*Beaker for weighing mice		*Beaker for weighing mice

Davebrid: added categories

2010-12-10T20:23:53Z

added categories

← Older revision		Revision as of 20:23, 10 December 2010
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	##Eject syringe.		##Eject syringe.
	#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein		#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein

			[[Category:Mouse Work]]
			[[Category:Glucose Homeostasis]]
			[[Category:Injections]]

Davebrid: added link to fasting insulin

2009-06-16T14:43:47Z

added link to fasting insulin

← Older revision		Revision as of 14:43, 16 June 2009
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	#Remove food from mice for about 6h. Add do not feed tag to cages.		#Remove food from mice for about 6h. Add do not feed tag to cages.
	#Bring mice to procedure room. Put water bottle on cage.		#Bring mice to procedure room. Put water bottle on cage.
	#Weigh mice, mark tails as necessary and take fasting glucose measurement.		#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capilary about 2/3 full with blood.
	#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).		#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
	#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.		#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.

Davebrid: added glucose strip ordering information

2009-06-10T13:41:32Z

added glucose strip ordering information

Davebrid at 15:07, 20 May 2009

2009-05-20T15:07:24Z

← Older revision		Revision as of 15:07, 20 May 2009
Line 1:		Line 1:
	==Materials (Bring Downstairs)==		==Materials (Bring Downstairs)==
	*Glucometer		*Glucometer - AccuChek Advantage
	*Glucose Test Strips		*Glucose Test Strips - AccuChek Comfort Curve
	*Scale		*Scale
	*Beaker for weighing mice		*Beaker for weighing mice
	*Syringes		*Syringes
	*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.		*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
			*Timer

	==Protocol==		==Protocol==
	#Remove food from mice for about 6h. Add do not feed tag to cages.		#Remove food from mice for about 6h. Add do not feed tag to cages.
	#Bring mice to procedure room.		#Bring mice to procedure room. Put water bottle on cage.
	#Weigh mice, mark tails as necessary and take fasting glucose measurement.		#Weigh mice, mark tails as necessary and take fasting glucose measurement.
	#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).		#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).

Davebrid: /* Protocol */

2009-05-13T15:42:51Z

Protocol

← Older revision		Revision as of 15:42, 13 May 2009
Line 18:		Line 18:
	:#Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over		:#Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
	:#Eject syringe.		:#Eject syringe.
			#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein

← Older revision		Revision as of 13:41, 10 June 2009
Line 1:		Line 1:
	==Materials (Bring Downstairs)==		==Materials (Bring Downstairs)==
	*Glucometer - AccuChek Advantage		*Glucometer - AccuChek Advantage
	*Glucose Test Strips - AccuChek Comfort Curve		*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]
	*Scale		*Scale
	*Beaker for weighing mice		*Beaker for weighing mice
Line 14:		Line 14:
	#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).		#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
	#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.		#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
	:#Immobilize mouse and restrain tail with one hand		##Immobilize mouse and restrain tail with one hand
	:#Aim needle between the midline and the hip bone		##Aim needle between the midline and the hip bone
	:#Insert syringe (do not inject) into cavity		##Insert syringe (do not inject) into cavity
	:#Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over		##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
	:#Eject syringe.		##Eject syringe.
	#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein		#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein