Genotyping Program - Revision history

Reddj: added are band size

2021-07-23T14:32:29Z

added are band size

← Older revision		Revision as of 14:32, 23 July 2021
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	\|[[TSC-floxed Genotyping\| TSC-floxed]]\|\| [[Cre PCR]] \|\| KO- 368bp, WT- 295bp \|\| aTSC, mTSC, Placenta TSC \|\| Two bands if heterozygote		\|[[TSC-floxed Genotyping\| TSC-floxed]]\|\| [[Cre PCR]] \|\| KO- 368bp, WT- 295bp \|\| aTSC, mTSC, Placenta TSC \|\| Two bands if heterozygote
	\|-		\|-
	\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)		\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| Between 340-400kDa \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)
	\|-		\|-
	\|[[SRY Genotyping\| SRY]]\|\| [[Cre PCR ]] \|\| Between 100-200kDa \|\| any mice too young to sex visually \|\| Only present in males		\|[[SRY Genotyping\| SRY]]\|\| [[Cre PCR ]] \|\| Between 100-200kDa \|\| any mice too young to sex visually \|\| Only present in males

Reddj: Added SRY

2021-07-23T14:31:13Z

Added SRY

← Older revision		Revision as of 14:31, 23 July 2021
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	\|-		\|-
	\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)		\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)
			\|-
			\|[[SRY Genotyping\| SRY]]\|\| [[Cre PCR ]] \|\| Between 100-200kDa \|\| any mice too young to sex visually \|\| Only present in males
	\|-		\|-
	\|[[Vac14 Genotyping\|Vac14]] \|\| [[Cre PCR]]\|\| \|\| \|\| Two bands if heterozygote		\|[[Vac14 Genotyping\|Vac14]] \|\| [[Cre PCR]]\|\| \|\| \|\| Two bands if heterozygote

Reddj at 15:00, 8 June 2020

2020-06-08T15:00:16Z

← Older revision		Revision as of 15:00, 8 June 2020
Line 6:		Line 6:
	\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)		\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)
	\|-		\|-
	\|[[Vac14 Genotyping\|Vac14]] \|\| [[Cre PCR]]\|\| \|\| Two bands if heterozygote		\|[[Vac14 Genotyping\|Vac14]] \|\| [[Cre PCR]]\|\| \|\| \|\| Two bands if heterozygote
	\|-		\|-
	\|[[TC10b Genotyping\|TC10b]] \|\| TC10b \|\| KO - 500bp, WT-1kb \|\| Two bands if heterozygote		\|[[TC10b Genotyping\|TC10b]] \|\| TC10b \|\| KO - 500bp, WT-1kb \|\| \|\| Two bands if heterozygote
	\|-		\|-
	\|[[Ob/ob_Genotyping\|ob/ob Genotyping]] \|\| ob/ob\|\| KO - 123bp, WT-104kb, control 191 bp \|\| Two reactions, see [[Ob/ob_Genotyping\|ob/ob Genotyping]]		\|[[Ob/ob_Genotyping\|ob/ob Genotyping]] \|\| ob/ob\|\| KO - 123bp, WT-104kb, control 191 bp \|\| \|\| Two reactions, see [[Ob/ob_Genotyping\|ob/ob Genotyping]]
	\|-		\|-
	\|[[MTM1 Genotyping\|MTM1]] \|\| MTM1 \|\| Mutant-575bp, WT-714bp \|\| Separate Reactions for WT and KO		\|[[MTM1 Genotyping\|MTM1]] \|\| MTM1 \|\| Mutant-575bp, WT-714bp \|\| \|\| Separate Reactions for WT and KO
	\|-		\|-
	\|[[CAP Genotyping\|CAP]] \|\| CAP \|\| \|\| Two bands if heterozygote, upper band for KO		\|[[CAP Genotyping\|CAP]] \|\| CAP \|\| \|\| \|\| Two bands if heterozygote, upper band for KO
	\|-		\|-
	\|[[Vinexin Genotyping\|Vinexin]] \|\| Vinexin \|\| \|\|		\|[[Vinexin Genotyping\|Vinexin]] \|\| Vinexin \|\| \|\| \|\|
	\|-		\|-
	\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| GR-KO and Adipo-GR-KO \|\| Two bands if heterozygote		\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| GR-KO and Adipo-GR-KO \|\| Two bands if heterozygote

Reddj: added relevant strains

2020-06-08T14:59:15Z

added relevant strains

← Older revision		Revision as of 14:59, 8 June 2020
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	{\| border="1"		{\| border="1"
	!\| Allele \|\| Program\|\| Fragment Size(s) \|\| Notes		!\| Allele \|\| Program\|\| Fragment Size(s) \|\| Strain(s) \|\| Notes
	\|-		\|-
	\|[[TSC-floxed Genotyping\| TSC-floxed]]\|\| [[Cre PCR]] \|\| KO- 368bp, WT- 295bp \|\| Two bands if heterozygote		\|[[TSC-floxed Genotyping\| TSC-floxed]]\|\| [[Cre PCR]] \|\| KO- 368bp, WT- 295bp \|\| aTSC, mTSC, Placenta TSC \|\| Two bands if heterozygote
	\|-		\|-
	\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)		\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)
	\|-		\|-
	\|[[Vac14 Genotyping\|Vac14]] \|\| [[Cre PCR]]\|\| \|\| Two bands if heterozygote		\|[[Vac14 Genotyping\|Vac14]] \|\| [[Cre PCR]]\|\| \|\| Two bands if heterozygote
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	\|[[Vinexin Genotyping\|Vinexin]] \|\| Vinexin \|\| \|\|		\|[[Vinexin Genotyping\|Vinexin]] \|\| Vinexin \|\| \|\|
	\|-		\|-
	\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| Two bands if heterozygote		\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| GR-KO and Adipo-GR-KO \|\| Two bands if heterozygote
	\|-		\|-
	\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- 540bp, WT- 400bp \|\| Two bands if heterozygote (run primers separately)		\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- 540bp, WT- 400bp \|\| mc4r \|\| Two bands if heterozygote (run primers separately)
	\|-		\|-
	\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- 574bp, WT- 678bp \|\| Two bands if heterozygote (run primers separately)		\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- 574bp, WT- 678bp \|\| ppp1r3c (PTG) \|\| Two bands if heterozygote (run primers separately)
	\|-		\|-
	\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)		\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp \|\| 4EBP1/2 \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
	\|-		\|-
	\|[[GDF15 Genotyping\| GDF15]] \|\| GDF15 \|\| KO- 200bp, WT- 600bp \|\| Two bands if heterozygote (run primers separately)		\|[[GDF15 Genotyping\| GDF15]] \|\| GDF15 \|\| KO- 200bp, WT- 600bp \|\| GDF15 \|\| Two bands if heterozygote (run primers separately)
	\|}		\|}

			AMPK, fTSC, fRaptor, and C57Bl6/J are all wild-type mice and don't need to be genotyped.
	[[ Category:Genotyping ]]		[[ Category:Genotyping ]]
	[[ Category:Mouse Work]]		[[ Category:Mouse Work]]

Reddj at 14:47, 8 June 2020

2020-06-08T14:47:21Z

← Older revision		Revision as of 14:47, 8 June 2020
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	\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- 574bp, WT- 678bp \|\| Two bands if heterozygote (run primers separately)		\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- 574bp, WT- 678bp \|\| Two bands if heterozygote (run primers separately)
	\|-		\|-
	\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)		\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
	\|-		\|-
	\|[[GDF15 Genotyping\| GDF15]] \|\| GDF15 \|\| KO- 200bp, WT- 600bp \|\| Two bands if heterozygote (run primers separately)		\|[[GDF15 Genotyping\| GDF15]] \|\| GDF15 \|\| KO- 200bp, WT- 600bp \|\| Two bands if heterozygote (run primers separately)

Reddj: added gdf15

2020-06-08T14:46:46Z

added gdf15

← Older revision		Revision as of 14:46, 8 June 2020
Line 25:		Line 25:
	\|-		\|-
	\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)		\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
			\|-
			\|[[GDF15 Genotyping\| GDF15]] \|\| GDF15 \|\| KO- 200bp, WT- 600bp \|\| Two bands if heterozygote (run primers separately)
	\|}		\|}

Reddj: added 4ebp1/2

2020-06-08T14:10:04Z

added 4ebp1/2

← Older revision		Revision as of 14:10, 8 June 2020
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	\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| Two bands if heterozygote		\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| Two bands if heterozygote
	\|-		\|-
	\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- ~~540~~, WT- ~~400~~ \|\| Two bands if heterozygote (run primers separately)		\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- 540bp, WT- 400bp \|\| Two bands if heterozygote (run primers separately)
	\|-		\|-
	\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- ~~574~~, WT- ~~678~~ \|\| Two bands if heterozygote (run primers separately)		\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- 574bp, WT- 678bp \|\| Two bands if heterozygote (run primers separately)
			\|-
			\|[[4EBP1/2 Genotyping\| 4EBP1/2]] \|\| [[Cre PCR]] \|\| 1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp \|\| Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
	\|}		\|}

Reddj: added PTG

2020-06-05T17:57:21Z

added PTG

← Older revision		Revision as of 17:57, 5 June 2020
Line 20:		Line 20:
	\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| Two bands if heterozygote		\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| Two bands if heterozygote
	\|-		\|-
	\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- 540, WT- 400 \|\| Two bands if heterozygote (run primers separately		\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- 540, WT- 400 \|\| Two bands if heterozygote (run primers separately)
			\|-
			\|[[ppp1r3c Genotyping\| PTG]] \|\| ppp1r3c (PTG) \|\| KO- 574, WT- 678 \|\| Two bands if heterozygote (run primers separately)
	\|}		\|}

Reddj at 17:41, 5 June 2020

2020-06-05T17:41:47Z

← Older revision		Revision as of 17:41, 5 June 2020
Line 9:		Line 9:
	\|-		\|-
	\|[[TC10b Genotyping\|TC10b]] \|\| TC10b \|\| KO - 500bp, WT-1kb \|\| Two bands if heterozygote		\|[[TC10b Genotyping\|TC10b]] \|\| TC10b \|\| KO - 500bp, WT-1kb \|\| Two bands if heterozygote
	\|		\|-
	\|[[Ob/ob_Genotyping\|ob/ob Genotyping]] \|\| ob/ob\|\| KO - 123bp, WT-104kb, control 191 bp \|\| Two reactions, see [[Ob/ob_Genotyping\|ob/ob Genotyping]]		\|[[Ob/ob_Genotyping\|ob/ob Genotyping]] \|\| ob/ob\|\| KO - 123bp, WT-104kb, control 191 bp \|\| Two reactions, see [[Ob/ob_Genotyping\|ob/ob Genotyping]]
	\|-		\|-

Reddj: added mc4r-Cone

2020-06-05T15:21:38Z

added mc4r-Cone

← Older revision		Revision as of 15:21, 5 June 2020
Line 2:		Line 2:
	!\| Allele \|\| Program\|\| Fragment Size(s) \|\| Notes		!\| Allele \|\| Program\|\| Fragment Size(s) \|\| Notes
	\|-		\|-
	\|[[TSC-floxed Genotyping\| TSC-floxed]]\|\| [[Cre PCR]] \|\| \|\| Two bands if heterozygote		\|[[TSC-floxed Genotyping\| TSC-floxed]]\|\| [[Cre PCR]] \|\| KO- 368bp, WT- 295bp \|\| Two bands if heterozygote
	\|-		\|-
	\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)		\|[[Cre Genotyping\|Cre]] \|\| [[Cre PCR ]] \|\| \|\| Only present if Transgene Positive (cannot detect homozygosity at transgene)
Line 9:		Line 9:
	\|-		\|-
	\|[[TC10b Genotyping\|TC10b]] \|\| TC10b \|\| KO - 500bp, WT-1kb \|\| Two bands if heterozygote		\|[[TC10b Genotyping\|TC10b]] \|\| TC10b \|\| KO - 500bp, WT-1kb \|\| Two bands if heterozygote
	\|-		\|
	\|[[Ob/ob_Genotyping\|ob/ob Genotyping]] \|\| ob/ob\|\| KO - 123bp, WT-104kb, control 191 bp \|\| Two reactions, see [[Ob/ob_Genotyping\|ob/ob Genotyping]]		\|[[Ob/ob_Genotyping\|ob/ob Genotyping]] \|\| ob/ob\|\| KO - 123bp, WT-104kb, control 191 bp \|\| Two reactions, see [[Ob/ob_Genotyping\|ob/ob Genotyping]]
	\|-		\|-
	\|[[MTM1 Genotyping\|MTM1]] \|\| MTM1 \|\| Mutant-~~575~~, WT-~~714~~ \|\| Separate Reactions for WT and KO		\|[[MTM1 Genotyping\|MTM1]] \|\| MTM1 \|\| Mutant-575bp, WT-714bp \|\| Separate Reactions for WT and KO
	\|-		\|-
	\|[[CAP Genotyping\|CAP]] \|\| CAP \|\| \|\| Two bands if heterozygote, upper band for KO		\|[[CAP Genotyping\|CAP]] \|\| CAP \|\| \|\| Two bands if heterozygote, upper band for KO
Line 18:		Line 18:
	\|[[Vinexin Genotyping\|Vinexin]] \|\| Vinexin \|\| \|\|		\|[[Vinexin Genotyping\|Vinexin]] \|\| Vinexin \|\| \|\|
	\|-		\|-
	\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- ~~300~~, WT- ~~247~~ \|\| Two bands if heterozygote		\|[[nr3c1 Genotyping\| GR KO]] \|\| nr3c1 \|\| KO- 300bp, WT- 247bp \|\| Two bands if heterozygote
			\|-
			\|[[mc4r-Cone Genotyping\| mc4r-Cone]] \|\| mc4r \|\| KO- 540, WT- 400 \|\| Two bands if heterozygote (run primers separately
	\|}		\|}


	[[ Category:Genotyping ]]		[[ Category:Genotyping ]]
	[[ Category:Mouse Work]]		[[ Category:Mouse Work]]