https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=French_Press 2026-06-01T22:05:03Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=French_Press&diff=85&oldid=prev 2009-05-06T16:14:22Z

<p>Changes to protocol based on the non-functionality of the sample loading aparatus. More specific information on loading the cylinder into the French Press added.</p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:14, 6 May 2009</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Prepare the Cell (Not Auto-Fill)==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Prepare the Cell (Not Auto-Fill)==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">#Invert cell on black stand without base piece.  Remove piston to fill line</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Pour in sample, replace base and place back on the press.  Ensure valves are tightened</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Pour in sample, replace base and place back on the press.  Ensure valves are tightened</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Loading Sample==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Loading Sample==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">#With the back valve open and the bottom valve closed insert tubing into sample</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>#Set French Press to Down and start. <ins style="font-weight: bold; text-decoration: none;">Pour sample into cylinder followed </ins>by the piston<ins style="font-weight: bold; text-decoration: none;">. </ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>#Set French Press to Down and start. <del style="font-weight: bold; text-decoration: none;"> Suction caused </del>by the piston <del style="font-weight: bold; text-decoration: none;">moving out of </del>the <del style="font-weight: bold; text-decoration: none;">cell will bring sample </del>into the <del style="font-weight: bold; text-decoration: none;">cell</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#Ensure that </ins>the <ins style="font-weight: bold; text-decoration: none;">piston is lubricated with glycerol. </ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">#When </del>the <del style="font-weight: bold; text-decoration: none;">base </del>is <del style="font-weight: bold; text-decoration: none;">at </del>the <del style="font-weight: bold; text-decoration: none;">bottom close </del>the <del style="font-weight: bold; text-decoration: none;">intake valve and stop the Press</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#Place cylinder with piston </ins>into the <ins style="font-weight: bold; text-decoration: none;">French Press instrument from the back, making sure that </ins>the <ins style="font-weight: bold; text-decoration: none;">piston's lever </ins>is <ins style="font-weight: bold; text-decoration: none;">orthogonal to </ins>the <ins style="font-weight: bold; text-decoration: none;">brace screws. </ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#Position </ins>the <ins style="font-weight: bold; text-decoration: none;">valves such that they are accessible to your hands.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Lysing Sample==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Lysing Sample==</div></td></tr> <!-- diff cache key bridgeslabproto-mw_:diff:1.41:old-57:rev-85:php=table --> </table>

Jpecherer https://bridgeslab.sph.umich.edu/protocols/index.php?title=French_Press&diff=57&oldid=prev 2009-05-04T12:22:02Z

<p>imported protocol</p> <p><b>New page</b></p><div>==Prepare the Cell (Not Auto-Fill)==<br /> #Invert cell on black stand without base piece. Remove piston to fill line<br /> #Pour in sample, replace base and place back on the press. Ensure valves are tightened<br /> <br /> ==Loading Sample==<br /> #With the back valve open and the bottom valve closed insert tubing into sample<br /> #Set French Press to Down and start. Suction caused by the piston moving out of the cell will bring sample into the cell<br /> #When the base is at the bottom close the intake valve and stop the Press<br /> <br /> ==Lysing Sample==<br /> #For bacteria 15 000 psi of cell pressure is sufficient. For a 1cm cell this corresponds to about 1100 psi on the gauge (see chart)<br /> #Turn black knob all the way to the left<br /> #Set Press to High and ensure both valves are securely closed<br /> #Start the press. The cell will move until it encounters pressure and will stop moving (the motor will still be running)<br /> #Slowly turn the knob until the gauge pressure reads desired pressure<br /> #Slowly open valve until sample comes out, keep an eye on the pressure and make sure it doesn’t drop. Continue until piston is fully depressed. Close bottom valve<br /> #Reload sample if necessary<br /> <br /> ==Cleaning/Storage==<br /> #Disassemble and rinse all parts with 70% ethanol then distilled water<br /> #Normally cells are stored at 4C to reduce sample heating</div>

Davebrid