https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Farnham_Lysis_Buffer
Farnham Lysis Buffer - Revision history
2026-05-30T23:22:25Z
Revision history for this page on the wiki
MediaWiki 1.45.1
https://bridgeslab.sph.umich.edu/protocols/index.php?title=Farnham_Lysis_Buffer&diff=984&oldid=prev
Davebrid: Wrote page for this buffer
2016-01-20T16:26:00Z
<p>Wrote page for this buffer</p>
<p><b>New page</b></p><div>==RIPA Buffer (for 50mL lysis buffer)==<br />
{| border="1"<br />
! || Final Concentration || per 50 mL || Stock || Location<br />
|- <br />
|PIPES pH 8.0 || 5 mM || 250 uL || 1M || 4C<br />
|- <br />
|KCl || 85 mM || 4.25 mL || 1M || Solutions Shelf<br />
|-<br />
| NP-40 || 0.5% || 0.357 mL || 70% || Chemical Shelf<br />
|- <br />
| Protease Inhibitors || || 1 mini tablet/1X || || 4C<br />
|}<br />
[[ Category:Buffer ]]<br />
[[ Category: ChIP ]]<br />
[[ Category: Immunoprecipitation ]]</div>
Davebrid