Culturing S2 Cells - Revision history

Davebrid at 13:23, 3 August 2009

2009-08-03T13:23:51Z

← Older revision		Revision as of 13:23, 3 August 2009
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	#Add 10 mL to fresh 10 cm dishes		#Add 10 mL to fresh 10 cm dishes
	#Add 1 mL of detached cells to each new plate		#Add 1 mL of detached cells to each new plate

			==Knockdown==
			*[[Designing and Preparing dsRNA]]
			*[[dsRNA Mediated Knockdown in S2 Cells]]

	==References==		==References==

2009-07-31T16:03:28Z

Materials

← Older revision		Revision as of 16:03, 31 July 2009
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	==Materials==		==Materials==
	*'''S2 cells''' ~~(available as frozen stocks from the Drosophila Genomics Resource Center or vendors)~~		*'''S2 cells''' see [[Freezing and Thawing S2 Cells]]
	*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs		*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs
	*'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs		*'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs

2009-07-31T16:00:38Z

← Older revision		Revision as of 16:00, 31 July 2009
Line 1:		Line 1:
	==Materials==		==Materials==
	*'''S2 cells''' (available as frozen stocks from the Drosophila Genomics Resource Center or vendors)		*'''S2 cells''' (available as frozen stocks from the Drosophila Genomics Resource Center or vendors)
	*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902)		*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs
	*'''Penicillin/streptomycin''' ~~100X~~ mix (Invitrogen, cat. no. 15240062)		*'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs
	*'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep (5 mL) into a tissue culture bottle		*'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep (10 mL) into a tissue culture bottle

	==Protocol==		==Protocol==

2009-07-31T15:43:56Z

← Older revision		Revision as of 15:43, 31 July 2009
Line 3:		Line 3:
	*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902)		*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902)
	*'''Penicillin/streptomycin''' 100X mix (Invitrogen, cat. no. 15240062)		*'''Penicillin/streptomycin''' 100X mix (Invitrogen, cat. no. 15240062)
			*'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep (5 mL) into a tissue culture bottle

			==Protocol==
			*Typically cells are split every 3-4 days at a ratio of 1:5 and grown at 25C
			*After 2-3 days the cells become slightly less adherent.
			#To split, gently aspirate the media (some cells will have detached from the plate surface)
			#Pipet a gentle stream of media over the surface of the plate (5 mL for normal passage)
			#Add 10 mL to fresh 10 cm dishes
			#Add 1 mL of detached cells to each new plate

	==References==		==References==
	see PMID 11752672 and PMID 18388942		see PMID 11752672 and PMID 18388942
	[[Category: Cell Culture]]		[[Category: Cell Culture]]
	[[Category: Drosophila]]		[[Category: Drosophila]]

2009-07-31T15:37:51Z

← Older revision		Revision as of 15:37, 31 July 2009
Line 1:		Line 1:
			==Materials==
			*'''S2 cells''' (available as frozen stocks from the Drosophila Genomics Resource Center or vendors)
			*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902)
			*'''Penicillin/streptomycin''' 100X mix (Invitrogen, cat. no. 15240062)
	==References==		==References==
	see PMID 11752672 and PMID 18388942		see PMID 11752672 and PMID 18388942
	[[Category: Cell Culture]]		[[Category: Cell Culture]]
	[[Category: Drosophila]]		[[Category: Drosophila]]
	~~[[Category: Knockdown]]~~

2009-07-31T15:35:48Z

2009-07-31T15:35:02Z

← Older revision		Revision as of 15:35, 31 July 2009
Line 1:		Line 1:
	==References==		==References==
	see ~~<pubmed>~~11752672~~</pubmed>~~ and ~~<pubmed>~~18388942~~</pubmed>~~		see PMID 11752672 and PMID 18388942

2009-07-31T15:34:27Z

initial page

New page

==References==
see <pubmed>11752672</pubmed> and <pubmed>18388942</pubmed>