https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Collecting_and_Storing_Mouse_SerumCollecting and Storing Mouse Serum - Revision history2026-05-30T22:39:30ZRevision history for this page on the wikiMediaWiki 1.45.1https://bridgeslab.sph.umich.edu/protocols/index.php?title=Collecting_and_Storing_Mouse_Serum&diff=2686&oldid=prevDavebrid: created serum page2024-01-18T15:54:15Z<p>created serum page</p>
<p><b>New page</b></p><div>==Materials==<br />
*Heparinzed capilaries (Fisher cat# 22-362574)<br />
<br />
==Protocol==<br />
#Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.<br />
#Transfer blood to labelled eppendorf tubes.<br />
#Let blood clot for 30 min on ice.<br />
#Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).<br />
#Gentry remove liquid layer and store at -80</div>Davebrid