https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Check_Clones_for_Correct_MutationCheck Clones for Correct Mutation - Revision history2026-06-01T22:13:56ZRevision history for this page on the wikiMediaWiki 1.45.1https://bridgeslab.sph.umich.edu/protocols/index.php?title=Check_Clones_for_Correct_Mutation&diff=143&oldid=prevDavebrid: wrote initial page2009-05-27T13:24:57Z<p>wrote initial page</p>
<p><b>New page</b></p><div>#Send for sequencing, preferably with primers reading both forward and reverse of the mutation<br />
#Go to http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi then click on tblastx on the tabs<br />
#Paste dna sequence in top box<br />
#Paste sequence, gi number or accession number for the refseq protein sequence into the lower box<br />
#Click BLAST<br />
#Look at the sequences and check for expected mutation<br />
#Check both forward and reverse sequences</div>Davebrid