Back-Crossing of Mouse Strains - Revision history

Davebrid: specified to keep backcrossing

2012-06-29T19:21:19Z

specified to keep backcrossing

← Older revision		Revision as of 19:21, 29 June 2012
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	==Notes==		==Notes==
	* As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can ~~eitehr~~ be back-crossed further or intercrossed for maintenance		* As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can either be back-crossed further or intercrossed for maintenance. It is better to keep back-crossing as many times as necessary.
	* '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased (see [[Ordering Mice]]) or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research.		* '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased (see [[Ordering Mice]]) or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research.
	* '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift.		* '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift.

Davebrid: added categories and link to ordering mice

2012-06-11T15:54:43Z

added categories and link to ordering mice

← Older revision		Revision as of 15:54, 11 June 2012
Line 1:		Line 1:
	==Notes==		==Notes==
	* As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can eitehr be back-crossed further or intercrossed for maintenance		* As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can eitehr be back-crossed further or intercrossed for maintenance
	* '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research.		* '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased (see [[Ordering Mice]]) or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research.
	* '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift.		* '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift.

	==Setting Up a Back-Cross==		==Setting Up a Back-Cross==
	* Generally we use female mice, and cross against a wild-type make since female mice are generally not used for experiments. Furthermore, since we cross against a pure male, then cross female pups to another pure male, the Y chromosome is always from the pure strain, and the X-chromosome is preferentially segregated to be from the pure strain.		* Generally we use female mice, and cross against a wild-type make since female mice are generally not used for experiments. Furthermore, since we cross against a pure male, then cross female pups to another pure male, the Y chromosome is always from the pure strain, and the X-chromosome is preferentially segregated to be from the pure strain.
	* When ordering mice order mice at 9 weeks of age, let them acclimatize to the facility for a week then set up the breeding cage at that point		* When [[Ordering Mice\|ordering mice]] order mice at 9 weeks of age, let them acclimatize to the facility for a week then set up the breeding cage at that point
	* For the next cross, generally take the first cohort of pups, and back-cross them again. This will make the process go faster.		* For the next cross, generally take the first cohort of pups, and back-cross them again. This will make the process go faster.

			[[Category: Mouse Work]]
			[[Category: Genotyping]]

Davebrid: wrote page for back-cross protocol

2012-06-11T15:44:53Z

wrote page for back-cross protocol

New page

==Notes==
* As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can eitehr be back-crossed further or intercrossed for maintenance
* '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research.
* '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift.

==Setting Up a Back-Cross==
* Generally we use female mice, and cross against a wild-type make since female mice are generally not used for experiments. Furthermore, since we cross against a pure male, then cross female pups to another pure male, the Y chromosome is always from the pure strain, and the X-chromosome is preferentially segregated to be from the pure strain.
* When ordering mice order mice at 9 weeks of age, let them acclimatize to the facility for a week then set up the breeding cage at that point
* For the next cross, generally take the first cohort of pups, and back-cross them again. This will make the process go faster.