https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=BDNF_PCR 2026-05-10T05:19:04Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=BDNF_PCR&diff=851&oldid=prev 2014-04-01T14:13:45Z

<p>Created page with &quot;&#039;&#039;&#039;BDNF Primers&#039;&#039;&#039; ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG...&quot;</p> <p><b>New page</b></p><div>&#039;&#039;&#039;BDNF Primers&#039;&#039;&#039;<br /> <br /> ----<br /> * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common)<br /> <br /> * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse)<br /> <br /> * oIMR01694 --- GGG AAC TTC CTG ACT AGG GG (Reverse)<br /> <br /> &#039;&#039;&#039;BDNF Cycler Program&#039;&#039;&#039;<br /> ----<br /> #94C - 10 minutes (HotStart Taq)<br /> #94C - 30 seconds<br /> #55C - 30 seconds<br /> #72C - 30 seconds ----- Repeat steps 2-4 for 35 cycles<br /> #72C - 2 minutes<br /> #4C - Hold<br /> ----<br /> http://jaxmice.jax.org/protocolsdb/f?p=116:2:3011685288607993::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:339,002266</div>

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