https://bridgeslab.sph.umich.edu/protocols/index.php?action=history&feed=atom&title=Analysing_Sequencing_Results_with_SeqMan 2026-06-02T02:43:07Z Revision history for this page on the wiki MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=Analysing_Sequencing_Results_with_SeqMan&diff=570&oldid=prev 2011-04-20T15:31:54Z

<p>wrote initial page</p> <p><b>New page</b></p><div># Open Seqman (under DNAStar Lasergene)<br /> # Download all sequence files from FTP site to a working folder (ftp://seqcore.brcf.med.umich.edu/)<br /> # In SeqMan go to Sequence -&gt; Add...<br /> # In your working folder select AB1 files and then Add then Done<br /> # If adding a reference sequence click Add Sequence... button then enter the nucleotide accession number and save this to your working folder.<br /> # Click Assemble button on the new window<br /> # A contig should be assembled on the window to the right. Double click on this.<br /> # Scan through the assembled contig to check for errors. Ignore errors only at ends of one sequencing run.<br /> # To see the chromatogram click on the grey triangle at the left of the sequence<br /> # Save the assembly as a SeqMan file.<br /> <br /> [[Category: Sequencing]]<br /> [[Category: Lasergene]]<br /> [[Category: Sequence Analysis]]<br /> [[Category: Bioinformatics]]</div>

Davebrid