https://bridgeslab.sph.umich.edu/protocols/api.php?action=feedcontributions&feedformat=atom&user=Pfeiferl 2026-04-19T18:20:14Z User contributions MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Tamoxifen&diff=1354 2017-06-20T16:49:04Z

<p>Pfeiferl: </p> <hr /> <div>[[ Category: SOP ]]<br /> [[ Category: Lab Safety]]<br /> '''Laboratory Director (LD) Approval is Required Prior to Performing this Procedure'''<br /> <br /> =Description=<br /> This standard operating procedure (SOP) outlines the handling and use of tamoxifen. Review this document and supply the information required in order to make it specific to your facility. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when handling tamoxifen.<br /> <br /> Tamoxifen is classified as a hazardous drug according to the National Institute for Occupational Safety and Health (NIOSH). Tamoxifen is a triphenyl ethylene derivative with marked anti-estrogen properties, which binds to estrogen receptors and, as a result, has proven useful in the treatment of breast cancer (antineoplastic). Tamoxifen citrate has been used to stimulate ovulation in infertility.<br /> <br /> Synonyms: Nolvadex, Istubal, Valodex<br /> <br /> =Potential Hazards=<br /> • May cause cancer.<br /> <br /> • May impair fertility and cause harm to the unborn child or breastfed babies.<br /> <br /> • Accidental ingestion of the material may be harmful.<br /> <br /> • May cause eye irritation.<br /> <br /> • This product may be harmful if inhaled and cause respiratory tract irritation. Persons with impaired respiratory function, airway diseases, and conditions such as emphysema or chronic bronchitis, may incur further disability if excessive concentrations of particulate are inhaled.<br /> <br /> • The material is not thought to be a skin irritant (as classified using animal models). But may be harmful if absorbed through the skin and may cause skin irritation; systemic effects may result following absorption. Entry into the blood-stream through, for example, cuts, abrasions or lesions, may produce systemic injury with harmful effects. Examine the skin prior to the use of the material and ensure that any external damage is suitably protected.<br /> <br /> =Engineering Controls=<br /> • Use a biological safety cabinet or chemical fume hood during reconstitution and dilution of this material.<br /> <br /> • Syringes used for tamoxifen injection must be safety engineered (self-sheathing syringes, luer-lock syringes, etc.)<br /> <br /> • Use with animals typically requires ABSL2 containment housing.<br /> <br /> • Animals should be appropriately restrained and/or sedated prior to administering injections and other dosing methods.<br /> <br /> • Laboratories and other spaces where handling of tamoxifen occurs must be equipped with an eyewash station.<br /> <br /> =Work Practice Controls=<br /> • Avoid inhalation.<br /> <br /> • Good hygiene practice requires that exposure be kept to a minimum and that suitable PPE and control measures be used in an occupational setting.<br /> <br /> • Decontamination should consist of surface cleaning with water and detergent followed by thorough rinsing. The use of detergent is recommended because there is no single accepted method of chemical deactivation for all agents involved. <br /> <br /> • A plastic backed absorbent pad should be placed under the work area during the process. This should be changed at the end of each procedure or when a spill occurs. <br /> <br /> =Personal Protective Equipment (PPE)=<br /> Appropriate lab attire including long pants and closed toe shoes must be worn. Personal protective equipment includes a lab coat or disposable coveralls, eye protection and two pairs of disposable nitrile gloves.  Gloves should be changed frequently and should be pulled up over sleeves if possible to reduce the likelihood of any exposed skin. Wash hands and arms immediately after working with the substance. <br /> <br /> Note: Respirators are masks designed to protect the wearer from specific airborne hazards and are different from surgical masks, which protect the wearer only from splashes and are primarily intended to protect others from infectious aerosols exhaled by the wearer. Respirator use requires employee participation in the Respiratory Protection Program, which involves medical clearance and annual fit testing and training. Please be clear about use of surgical masks versus respirators. (Do NOT use the vague term “masks”.)<br /> <br /> =Transportation and Storage=<br /> • Keep containers tightly closed and in dry, well-ventilated area.<br /> <br /> • Keep away from light. <br /> <br /> • Avoid strong oxidizing agents.<br /> <br /> • Tamoxifen is regulated by DOT as a Hazardous Material as well as IATA for Transport of Dangerous Goods.<br /> <br /> =Waste Disposal=<br /> All hazardous chemical waste should be placed in an appropriate container for disposal (pail or bottle). The container should be closed except when actively adding waste. The container must be located in the area where hazardous drugs are being used. <br /> <br /> All items contaminated by hazardous drugs, including gloves, syringes, vials needles, animal bedding and solution containers should be disposed according to OSEH guidelines.<br /> <br /> • Sharps – place needles, syringes with needles attached and other breakable items into appropriately labeled sharps containers. <br /> <br /> • Empty stock vials, reagent bottles, etc. – triple rinse with copious amounts of water. Deface label with black magic marker or scraper. Place in a cardboard box for disposal.<br /> <br /> Because most spent, unused and expired chemicals/materials are considered hazardous wastes, they must be properly disposed of. Do not dispose of chemical wastes by dumping them down a sink, flushing in a toilet or discarding in regular trash containers, unless authorized by OSEH Hazardous Materials Management (HMM). Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information.<br /> <br /> =Exposures/Unintended Contact=<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> <br /> ==SWALLOWED==<br /> • Rinse mouth with water. <br /> <br /> • Consult a physician. <br /> <br /> ==EYE==<br /> If this product comes in contact with the eyes: <br /> • Wash out immediately with fresh running water for a minimum of 15 minutes. <br /> <br /> • Ensure complete irrigation of the eye by keeping eyelids apart and away from eye and moving the eyelids by occasionally lifting the upper and lower lids. <br /> <br /> • If pain persists or recurs seek medical attention. <br /> <br /> • Removal of contact lenses after an eye injury should only be undertaken by skilled personnel.<br /> <br /> ==SKIN==<br /> If skin contact occurs: <br /> • Immediately remove all contaminated clothing, including footwear.<br /> <br /> • Flush skin and hair with running water (and soap if available) for a minimum of 15 minutes. <br /> <br /> • Seek medical attention in event of irritation.<br /> <br /> ==INHALED==<br /> • Move person from contaminated area into fresh air. <br /> <br /> • Move person into fresh air.<br /> <br /> • Seek medical attention.<br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to Work Connections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the Work Connections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> ===TREATMENT FACILITIES:===<br /> U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> <br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> <br /> University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> <br /> UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> <br /> =Spill Procedure=<br /> • When a spill occurs, personal safety should always come first.<br /> <br /> • Alert and clear everyone in the immediate area where the spill occurred. <br /> <br /> A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., (less than 1 Gallon or 3.5 Liters). A major/large chemical spill requires active assistance from emergency personnel.<br /> <br /> Spill Response Steps:<br /> <br /> ===MINOR CHEMICAL SPILL===<br /> • Alert people in immediate area of spill.<br /> <br /> • Open outside windows, if possible.<br /> <br /> • Use proper personal protective equipment (PPE) as indicated above.<br /> <br /> • Avoid breathing vapors from spill.<br /> <br /> • Confine spill to as small an area as possible.<br /> <br /> • Do not wash spill down the drain.<br /> <br /> • Use appropriate spill kits/sorbents to absorb spill. Collect contaminated materials and residues and place in container. For powdered chemicals sweep carefully to avoid generation of dust or, if appropriate, use moist sorbent pads or wet the powder with a suitable solvent and then wipe with a dry cloth. Contact OSEH-HMM (734) 763-4568 for proper disposal.<br /> <br /> • Clean spill area with water.<br /> <br /> ===MAJOR CHEMICAL SPILL===<br /> Report large chemical spills (greater than 1 Gallon or 3.5 Liters) in corridors or common areas, e.g., hallways, elevators, eating areas, rest rooms, offices, etc., to University of Michigan Division of Public Safety and Security (DPSS) at 911.<br /> • Attend to injured or contaminated persons and remove them from exposure.<br /> <br /> • Alert people in the laboratory to evacuate.<br /> <br /> • Call University of Michigan Division of Public Safety and Security (DPSS) at 911 immediately for assistance.<br /> <br /> • Close doors to affected area.<br /> <br /> • Post warnings to keep people from entering the area.<br /> <br /> • Have person available that has knowledge of incident and laboratory to assist emergency personnel.<br /> <br /> Additional Spill Links:<br /> • http://oseh.umich.edu/hazardous-waste/spill-response/ <br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> =Training of Personnel=<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling tamoxifen.<br /> <br /> =Certification=<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure.<br /> <br /> *[[User:Iharvey|Iharvey]] ([[User talk:Iharvey|talk]]) 20:56, 10 November 2016 (UTC)<br /> * [[User:Elhabbal|Elhabbal]] ([[User talk:Elhabbal|talk]]) 22:23, 12 June 2017 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 16:48, 20 June 2017 (UTC)<br /> <br /> Laboratory Director Revision Date <br /> �<br /> Major Revisions (Tracking purposes only – Do not print as part</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Tamoxifen&diff=1353 2017-06-20T16:48:46Z

<p>Pfeiferl: </p> <hr /> <div>[[ Category: SOP ]]<br /> [[ Category: Lab Safety]]<br /> '''Laboratory Director (LD) Approval is Required Prior to Performing this Procedure'''<br /> <br /> =Description=<br /> This standard operating procedure (SOP) outlines the handling and use of tamoxifen. Review this document and supply the information required in order to make it specific to your facility. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when handling tamoxifen.<br /> <br /> Tamoxifen is classified as a hazardous drug according to the National Institute for Occupational Safety and Health (NIOSH). Tamoxifen is a triphenyl ethylene derivative with marked anti-estrogen properties, which binds to estrogen receptors and, as a result, has proven useful in the treatment of breast cancer (antineoplastic). Tamoxifen citrate has been used to stimulate ovulation in infertility.<br /> <br /> Synonyms: Nolvadex, Istubal, Valodex<br /> <br /> =Potential Hazards=<br /> • May cause cancer.<br /> <br /> • May impair fertility and cause harm to the unborn child or breastfed babies.<br /> <br /> • Accidental ingestion of the material may be harmful.<br /> <br /> • May cause eye irritation.<br /> <br /> • This product may be harmful if inhaled and cause respiratory tract irritation. Persons with impaired respiratory function, airway diseases, and conditions such as emphysema or chronic bronchitis, may incur further disability if excessive concentrations of particulate are inhaled.<br /> <br /> • The material is not thought to be a skin irritant (as classified using animal models). But may be harmful if absorbed through the skin and may cause skin irritation; systemic effects may result following absorption. Entry into the blood-stream through, for example, cuts, abrasions or lesions, may produce systemic injury with harmful effects. Examine the skin prior to the use of the material and ensure that any external damage is suitably protected.<br /> <br /> =Engineering Controls=<br /> • Use a biological safety cabinet or chemical fume hood during reconstitution and dilution of this material.<br /> <br /> • Syringes used for tamoxifen injection must be safety engineered (self-sheathing syringes, luer-lock syringes, etc.)<br /> <br /> • Use with animals typically requires ABSL2 containment housing.<br /> <br /> • Animals should be appropriately restrained and/or sedated prior to administering injections and other dosing methods.<br /> <br /> • Laboratories and other spaces where handling of tamoxifen occurs must be equipped with an eyewash station.<br /> <br /> =Work Practice Controls=<br /> • Avoid inhalation.<br /> <br /> • Good hygiene practice requires that exposure be kept to a minimum and that suitable PPE and control measures be used in an occupational setting.<br /> <br /> • Decontamination should consist of surface cleaning with water and detergent followed by thorough rinsing. The use of detergent is recommended because there is no single accepted method of chemical deactivation for all agents involved. <br /> <br /> • A plastic backed absorbent pad should be placed under the work area during the process. This should be changed at the end of each procedure or when a spill occurs. <br /> <br /> =Personal Protective Equipment (PPE)=<br /> Appropriate lab attire including long pants and closed toe shoes must be worn. Personal protective equipment includes a lab coat or disposable coveralls, eye protection and two pairs of disposable nitrile gloves.  Gloves should be changed frequently and should be pulled up over sleeves if possible to reduce the likelihood of any exposed skin. Wash hands and arms immediately after working with the substance. <br /> <br /> Note: Respirators are masks designed to protect the wearer from specific airborne hazards and are different from surgical masks, which protect the wearer only from splashes and are primarily intended to protect others from infectious aerosols exhaled by the wearer. Respirator use requires employee participation in the Respiratory Protection Program, which involves medical clearance and annual fit testing and training. Please be clear about use of surgical masks versus respirators. (Do NOT use the vague term “masks”.)<br /> <br /> =Transportation and Storage=<br /> • Keep containers tightly closed and in dry, well-ventilated area.<br /> <br /> • Keep away from light. <br /> <br /> • Avoid strong oxidizing agents.<br /> <br /> • Tamoxifen is regulated by DOT as a Hazardous Material as well as IATA for Transport of Dangerous Goods.<br /> <br /> =Waste Disposal=<br /> All hazardous chemical waste should be placed in an appropriate container for disposal (pail or bottle). The container should be closed except when actively adding waste. The container must be located in the area where hazardous drugs are being used. <br /> <br /> All items contaminated by hazardous drugs, including gloves, syringes, vials needles, animal bedding and solution containers should be disposed according to OSEH guidelines.<br /> <br /> • Sharps – place needles, syringes with needles attached and other breakable items into appropriately labeled sharps containers. <br /> <br /> • Empty stock vials, reagent bottles, etc. – triple rinse with copious amounts of water. Deface label with black magic marker or scraper. Place in a cardboard box for disposal.<br /> <br /> Because most spent, unused and expired chemicals/materials are considered hazardous wastes, they must be properly disposed of. Do not dispose of chemical wastes by dumping them down a sink, flushing in a toilet or discarding in regular trash containers, unless authorized by OSEH Hazardous Materials Management (HMM). Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information.<br /> <br /> =Exposures/Unintended Contact=<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> <br /> ==SWALLOWED==<br /> • Rinse mouth with water. <br /> <br /> • Consult a physician. <br /> <br /> ==EYE==<br /> If this product comes in contact with the eyes: <br /> • Wash out immediately with fresh running water for a minimum of 15 minutes. <br /> <br /> • Ensure complete irrigation of the eye by keeping eyelids apart and away from eye and moving the eyelids by occasionally lifting the upper and lower lids. <br /> <br /> • If pain persists or recurs seek medical attention. <br /> <br /> • Removal of contact lenses after an eye injury should only be undertaken by skilled personnel.<br /> <br /> ==SKIN==<br /> If skin contact occurs: <br /> • Immediately remove all contaminated clothing, including footwear.<br /> <br /> • Flush skin and hair with running water (and soap if available) for a minimum of 15 minutes. <br /> <br /> • Seek medical attention in event of irritation.<br /> <br /> ==INHALED==<br /> • Move person from contaminated area into fresh air. <br /> <br /> • Move person into fresh air.<br /> <br /> • Seek medical attention.<br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to Work Connections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the Work Connections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> ===TREATMENT FACILITIES:===<br /> U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> <br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> <br /> University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> <br /> UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> <br /> =Spill Procedure=<br /> • When a spill occurs, personal safety should always come first.<br /> <br /> • Alert and clear everyone in the immediate area where the spill occurred. <br /> <br /> A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., (less than 1 Gallon or 3.5 Liters). A major/large chemical spill requires active assistance from emergency personnel.<br /> <br /> Spill Response Steps:<br /> <br /> ===MINOR CHEMICAL SPILL===<br /> • Alert people in immediate area of spill.<br /> <br /> • Open outside windows, if possible.<br /> <br /> • Use proper personal protective equipment (PPE) as indicated above.<br /> <br /> • Avoid breathing vapors from spill.<br /> <br /> • Confine spill to as small an area as possible.<br /> <br /> • Do not wash spill down the drain.<br /> <br /> • Use appropriate spill kits/sorbents to absorb spill. Collect contaminated materials and residues and place in container. For powdered chemicals sweep carefully to avoid generation of dust or, if appropriate, use moist sorbent pads or wet the powder with a suitable solvent and then wipe with a dry cloth. Contact OSEH-HMM (734) 763-4568 for proper disposal.<br /> <br /> • Clean spill area with water.<br /> <br /> ===MAJOR CHEMICAL SPILL===<br /> Report large chemical spills (greater than 1 Gallon or 3.5 Liters) in corridors or common areas, e.g., hallways, elevators, eating areas, rest rooms, offices, etc., to University of Michigan Division of Public Safety and Security (DPSS) at 911.<br /> • Attend to injured or contaminated persons and remove them from exposure.<br /> <br /> • Alert people in the laboratory to evacuate.<br /> <br /> • Call University of Michigan Division of Public Safety and Security (DPSS) at 911 immediately for assistance.<br /> <br /> • Close doors to affected area.<br /> <br /> • Post warnings to keep people from entering the area.<br /> <br /> • Have person available that has knowledge of incident and laboratory to assist emergency personnel.<br /> <br /> Additional Spill Links:<br /> • http://oseh.umich.edu/hazardous-waste/spill-response/ <br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> =Training of Personnel=<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling tamoxifen.<br /> <br /> =Certification=<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure.<br /> <br /> *[[User:Iharvey|Iharvey]] ([[User talk:Iharvey|talk]]) 20:56, 10 November 2016 (UTC)<br /> * [[User:Elhabbal|Elhabbal]] ([[User talk:Elhabbal|talk]]) 22:23, 12 June 2017 (UTC)<br /> [[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 16:48, 20 June 2017 (UTC)<br /> Laboratory Director Revision Date <br /> �<br /> Major Revisions (Tracking purposes only – Do not print as part</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=Triglyceride_Assay_from_Cells_and_Tissues&diff=1270 2017-06-07T17:05:21Z

<p>Pfeiferl: </p> <hr /> <div>==Materials==<br /> * '''Homogenization Buffer''' (50 mM Tris pH 8, 5 mM EDTA, 30 mM Mannitol, PI inhibitor, can be made in bulk without the PI, PI added fresh)<br /> * 10M KOH<br /> * '''Chloroform/Methanol Mixture''' (2:1)<br /> * '''Butanol Mixture''': 3 mL butanol, 1.66 mL Triton-X114, 0.33 mL Methanol<br /> * Sigma Triglyceride Assay Kit (Cat TR0100)<br /> <br /> ==Protocol==<br /> #Weigh out 30-50mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube (2 mL). Add one stainless steel ball bearing.<br /> #Add 500ul Homogenization Buffer<br /> #Homogenize with Qiagen Tissue Lyser for 3 minutes @ 25Hz for Liver/WAT or for 5 minutes @ 30Hz for muscle<br /> #Add 12.5ul KOH<br /> #Mix by inverting then transfewr sample to a new 1.5 mL tube. Place dirty ball bearing in ethanol (located in the fume hood)<br /> #Add 800ul '''Chloroform/Methanol Mixture'''<br /> #Vortex vigorously then sit at room temperature for 5 minutes<br /> #Centrifuge for 10 minutes @ 13000G<br /> #Transfer 400 ul of the bottom layer into a new tube<br /> #Let evaporate overnight at room temperature<br /> #Add'''(50ul)''' of '''Butanol Mixture''' and vortex. See Suggested Volumes for your specific tissue.<br /> #Measure triglyceride levels using SIgma Diagnostic Kit, using 5ul of sample.<br /> ##Resuspend triglyceride and glycerol reagent with water if necessary<br /> ##Calculate how many sample you have (samples + blank + standard curve)<br /> ##Prepare reagent. You need 560ul '''(80ul)''' of glycerol reagent and 140ul '''(20ul)''' of triglyceride reagent. Make extra and combine in a Falcon tube.<br /> ##Aliquot '''100ul into a well of a 96 well plate'''<br /> ##For standards, add 0-5 and .5ul of glycerol standard<br /> ##Add 5ul of resuspended lipid to each well to start (also make a 5ul blank of the butanol mixture) and mix.<br /> ##Pop any bubbles with tip before incubating.<br /> ##Let sit for ~30 mins @ room temperature (or 5mins @ 37C if you are in a hurry). If using &gt;10ul of butanol mix the solution may be cloudy. Let it settle and it should become more clear.<br /> ##Measure absorbance @ 540nm<br /> ##If any samples are A540&lt;0.1 or above the 5ul standard the A540, then repeat with more or less lipid as required.</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=Triglyceride_Assay_from_Cells_and_Tissues&diff=1269 2017-06-07T17:04:46Z

<p>Pfeiferl: </p> <hr /> <div>==Materials==<br /> * '''Homogenization Buffer''' (50 mM Tris pH 8, 5 mM EDTA, 30 mM Mannitol, PI inhibitor, can be made in bulk without the PI, PI added fresh)<br /> * 10M KOH<br /> * '''Chloroform/Methanol Mixture''' (2:1)<br /> * '''Butanol Mixture''': 3 mL butanol, 1.66 mL Triton-X114, 0.33 mL Methanol<br /> * Sigma Triglyceride Assay Kit (Cat TR0100)<br /> <br /> ==Protocol==<br /> #Weigh out 30-50mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube (2 mL). Add one stainless steel ball bearing.<br /> #Add 500ul Homogenization Buffer<br /> #Homogenize with Qiagen Tissue Lyser for 3 minutes @ 25Hz for Liver/WAT or for 5 minutes @ 30Hz for muscle<br /> #Add 12.5ul KOH<br /> #Mix by inverting then transfewr sample to a new 1.5 mL tube. Place dirty ball bearing in ethanol (located in the fume hood)<br /> #Add 800ul '''Chloroform/Methanol Mixture'''<br /> #Vortex vigorously then sit at room temperature for 5 minutes<br /> #Centrifuge for 10 minutes @ 13000G<br /> #Transfer 400 ul of the bottom layer into a new tube<br /> #Let evaporate overnight at room temperature<br /> #Add'''(50ul)''' of '''Butanol Mixture'''. See Suggested Volumes for your specific tissue.<br /> #Measure triglyceride levels using SIgma Diagnostic Kit, using 5ul of sample.<br /> ##Resuspend triglyceride and glycerol reagent with water if necessary<br /> ##Calculate how many sample you have (samples + blank + standard curve)<br /> ##Prepare reagent. You need 560ul '''(80ul)''' of glycerol reagent and 140ul '''(20ul)''' of triglyceride reagent. Make extra and combine in a Falcon tube.<br /> ##Aliquot '''100ul into a well of a 96 well plate'''<br /> ##For standards, add 0-5 and .5ul of glycerol standard<br /> ##Add 5ul of resuspended lipid to each well to start (also make a 5ul blank of the butanol mixture) and mix.<br /> ##Pop any bubbles with tip before incubating.<br /> ##Let sit for ~30 mins @ room temperature (or 5mins @ 37C if you are in a hurry). If using &gt;10ul of butanol mix the solution may be cloudy. Let it settle and it should become more clear.<br /> ##Measure absorbance @ 540nm<br /> ##If any samples are A540&lt;0.1 or above the 5ul standard the A540, then repeat with more or less lipid as required.</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=Triglyceride_Assay_from_Cells_and_Tissues&diff=1268 2017-06-07T17:04:27Z

<p>Pfeiferl: </p> <hr /> <div>==Materials==<br /> * '''Homogenization Buffer''' (50 mM Tris pH 8, 5 mM EDTA, 30 mM Mannitol, PI inhibitor, can be made in bulk without the PI, PI added fresh)<br /> * 10M KOH<br /> * '''Chloroform/Methanol Mixture''' (2:1)<br /> * '''Butanol Mixture''': 3 mL butanol, 1.66 mL Triton-X114, 0.33 mL Methanol<br /> * Sigma Triglyceride Assay Kit (Cat TR0100)<br /> <br /> ==Protocol==<br /> #Weigh out 30-50mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube (2 mL). Add one stainless steel ball bearing.<br /> #Add 500ul Homogenization Buffer<br /> #Homogenize with Qiagen Tissue Lyser for 3 minutes @ 25Hz for Liver/WAT or for 5 minutes @ 30Hz for muscle<br /> #Add 12.5ul KOH<br /> #Mix by inverting then transfewr sample to a new 1.5 mL tube. Place dirty ball bearing in ethanol (located in the fume hood)<br /> #Add 800ul '''Chloroform/Methanol Mixture'''<br /> #Vortex vigorously then sit at room temperature for 5 minutes<br /> #Centrifuge for 10 minutes @ 13000G<br /> #Transfer 400 ul of the bottom layer into a new tube<br /> #Let evaporate overnight at room temperature<br /> #Add'''(50ul)''' of '''Butanol Mixture'''. See Suggested Volumes for your specific tissue.<br /> #Measure triglyceride levels using SIgma Diagnostic Kit, using 5ul of sample.<br /> ##Resuspend triglyceride and glycerol reagent with water if necessary<br /> ##Calculate how many sample you have (samples + blank + standard curve)<br /> ##Prepare reagent. You need 560ul '''(80ul)''' of glycerol reagent and 140ul '''(20ul)''' of triglyceride reagent. Make extra and combine in a Falcon tube.<br /> ##Aliquot '''100ul into a well of a 96 well plate'''<br /> ##For standards, add 0-5 and .5ul of glycerol standard<br /> ##Add 5ul of resuspended lipid to each well to start (also make a 5ul blank of the butanol mixture) and mix.<br /> Pop any bubbles with tip before incubating.<br /> ##Let sit for ~30 mins @ room temperature (or 5mins @ 37C if you are in a hurry). If using &gt;10ul of butanol mix the solution may be cloudy. Let it settle and it should become more clear.<br /> ##Measure absorbance @ 540nm<br /> ##If any samples are A540&lt;0.1 or above the 5ul standard the A540, then repeat with more or less lipid as required.</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Autoclave&diff=1266 2017-06-05T18:11:59Z

<p>Pfeiferl: </p> <hr /> <div>[[Category: SOP]]<br /> [[Category: Lab Safety]]<br /> <br /> ==Description==<br /> This standard operating procedure outlines the use of autoclaves<br /> Autoclaves are used to sterilize equipment through a process that incorporates high pressure and high temperature.<br /> <br /> ==Potential Hazards==<br /> Autoclaves pose several potential hazards to labs who frequently use them. Among the physical hazards are heat, steam, and high pressure. Biological hazards, including improperly autoclaved infectious materials, are also present. <br /> <br /> Controls for different brands of autoclaves may have their own unique characteristics for loading, load sizes, cycle types and cycle settings. The type of materials to be sterilized will determine the sterilization cycle that will be used. For this reason it is important to read and understand the user’s manual for the specific model of autoclave that is being used prior to first time use. Always ensure that the user’s manual is readily available in case questions or concerns arise during the operation of the autoclave.<br /> <br /> ==Engineering Controls==<br /> Autoclave monitoring and maintenance is an important aspect of a safe and properly functioning autoclave. Follow the manufacturer’s recommendations for preventive maintenance and ensure that all contractors hired to perform regular maintenance and repairs are approved by the manufacturer. Autoclave operators shall ensure that each autoclave is monitored as follows:<br /> <br /> Heat Sensitive Tape Monitoring – Operators shall use heat sensitive sterilization indicator tape for each load to indicate that the load has undergone an effective steam sterilization process.<br /> * Change on autoclave tape means the proper temperature was achieved, but does not mean that temperature was maintained for the proper length of time. <br /> * Place a small piece of autoclave tape on each bottle and tip box.<br /> Biological Indicators – Operators who autoclave medical/biohazardous waste need to do the following:<br /> * At least once a month use a biological indicator such as Bacillus Stearothermophilus (e.g. Prospore2) placed at the center of a load processed under standard operating conditions to confirm the attainment of adequate sterilization conditions.<br /> ==Work Practice Controls==<br /> Do not autoclave items containing corrosives, solvents, volatiles or radioactive materials.<br /> <br /> ===Prior to loading===<br /> * Before using the autoclave, be sure previous users have cleared the autoclave, and that no broken items have been left inside.<br /> * Check to be sure the drain is clear.<br /> * Verify that the interior of the autoclave is in usable condition. <br /> <br /> ===Loading===<br /> * Carefully load items to be autoclaved, DO NOT overload the autoclave.<br /> * Bottles containing liquids should be placed inside a heat resistant plastic tray (secondary container) containing an inch of water.<br /> * Bottles should not be filled more than 2/3.<br /> * Keep 1-2 inches of space between bottles.<br /> * DO NOT tighten caps on bottles fully.<br /> * Individual glassware pieces should be within a secondary container on a shelf or rack and never placed directly on the autoclave chamber bottom or floor.<br /> * Make sure that the door of the autoclave is fully closed and latched and ensure that the correct cycle for the items being autoclaved has been selected before starting the cycle.<br /> <br /> ===Opening===<br /> * Wear the proper PPE, including autoclave gloves and close toed shoes when opening the autoclave door after a cycle. <br /> * When the cycle is complete, open the door slowly to minimize exposure to steam. Keep appendages away from the opening.<br /> <br /> ===When autoclaving liquids:===<br /> * When running an autoclave cycle with liquids, choose the liquid cycle setting. <br /> * To prevent bottles from shattering during the pressurization, the caps of containers with liquids must be loosened before loading.<br /> * Use only borosilicate glass (Pyrex™ or Kimax™).<br /> * Use a secondary container with a solid bottom and walls to contain the contents and catch spills.<br /> * Set a timer to come and unload the autoclave, to ensure it is available for use by other labs.<br /> * Wait 10 minutes after the cycle ends before removing autoclaved liquid load items.<br /> * Let the liquids stand for at least a full hour before touching with ungloved hands. Be sure to let others in the area know that a heat hazard is present.<br /> <br /> ===When autoclaving dry loads:=== <br /> * Add ¼ to ½ inch of water to the tray so that the bottles will heat evenly. Do not fully tighten bottle caps, this could lead to the bottle shattering. Loose caps allow in inside of the bottle to be sterilized.<br /> * Check plastic materials to ensure that they are compatible with being autoclaved.<br /> * Choose the dry porous materials cycle.<br /> * Set a timer to come and unload the autoclave, to ensure it is available for use by other labs.<br /> * Before removing autoclaved items, wait 5 minutes after the cycle ends for loads containing only dry glassware.<br /> * For dry loads, let the glassware cool for a minimum of 15 minutes before touching it with ungloved hands.<br /> <br /> ==Protective Equipment (PPE)==<br /> Wear the proper PPE, including heat resistant gloves, and close toed shoes when opening the autoclave door after a cycle. <br /> ==Waste Disposal==<br /> Place waste in a university approved autoclave bag; avoid over packing of autoclave bags. Do not seal bags or close bottles and other containers tightly. After sterilization cycle, close and seal bag. The sterilized waste can be transported directly to the dumpster by Building Services or laboratory staff without boxing or labeling. Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information. <br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> <br /> Discontinue use immediately if an autoclave is not working properly. Post a sign alerting others not to use the autoclave. Mechanical failures need to be attended to by a trained technician, DO NOT try to fix it yourself. Contact facilities to have it repaired.<br /> If you are burned, you should seek medical treatment immediately. Burns to the face, third- degree burns or burns over large areas of the body should be treated as emergencies. (Call 911). Minor burns should be treated by using first aid procedures, including immersing the burn in cool water immediately, removing clothing from the burn area and keeping the injured area cool for at least 5 minutes. Regardless of the severity, notify your Laboratory Director/Supervisor!<br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> ====TREATMENT FACILITIES:====<br /> ====U-M Occupational Health Services -- Campus Employees====<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> ====University Health Services -- University students (non-life threatening conditions)====<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> ====UMHS Emergency Department -- after clinic hours or on weekends====<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> Click here for more information.<br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> ==Training of personnel==<br /> All personnel are required to complete the Autoclave Safety training online through MyLINC (Course BLS013w). All users shall be trained before operating an autoclave; the Laboratory Director/Supervisor is responsible for ensuring that each person in the lab is appropriately trained. All training must be documented and the records maintained in the lab with other safety training certificates. The Laboratory Director/Supervisor is encouraged to use this policy as a guide for training new personnel.<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> *[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:13, 19 October 2016 (UTC)<br /> * [[User:Davebridges|Davebridges]] ([[User talk:Davebridges|talk]]) 14:53, 8 November 2016 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:11, 5 June 2017 (UTC)<br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? X YES ☐ NO<br /> Laboratory Director - Dave Bridges Revision Date - 2016-10-19</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Flammables_and_Combustibles&diff=1265 2017-06-05T18:11:26Z

<p>Pfeiferl: </p> <hr /> <div>[[ Category: SOP ]]<br /> [[ Category: Lab Safety ]]<br /> <br /> ==Description==<br /> This standard operating procedure outlines the handling and use of flammable and combustible materials. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when handling flammable and combustible materials.<br /> <br /> Flammable and combustible liquids can burn and are classified, or grouped, as either flammable or combustible by their flashpoints. Flammable liquids will ignite and burn easily at normal working temperatures. Combustible liquids have the ability to burn at temperatures that are usually above working temperatures. Flammable liquids have a flashpoint below 37.8°C (100°F) while Combustible liquids have a flashpoint at or above 37.8°C (100°F) and below 93.3°C (200°F).<br /> <br /> ==Potential Hazards==<br /> Flammability - Check Safety Data Sheet of the material for other hazards. <br /> Definitions – <br /> * Flammable gas – a gas that, at 68°F or less and standard pressure, forms a flammable mixture with air at a concentration of 13% by volume or less OR that, at ambient temperature and pressure, forms a range of flammable mixtures with air wider than 12% by volume, regardless of the lower limit.<br /> * Flammable liquid – a liquid having a flash point below 100°F. <br /> * Flammable solid – a solid, other than a blasting agent or explosive, that (1) has an ignition temperature below 212°F, or (2) is capable of causing a fire through friction, absorption of moisture, or spontaneous chemical change, or (3) burns so vigorously and persistently as to create a serious hazard.<br /> * Combustible liquid – a liquid having a flash point at or above 100°F.<br /> <br /> ==Engineering Controls==<br /> Work in a chemical fume hood if air concentrations above 10% of the lower flammable limit may be reached, if the chemical is irritating to the eyes and respiratory system, and/or is toxic by inhalation.<br /> <br /> ==Work Practice Controls==<br /> * Alert others in your lab of the materials you will be working with.<br /> * Review SDSs before working with material.<br /> * Know the location of the nearest fire extinguisher before beginning work.<br /> * Use in the smallest practical quantities for the experiment being performed.<br /> * Avoid using ignition sources (Bunsen burners, hot plates, electrical equipment with frayed or cracked wiring, etc.) and/or creating static electricity in areas where highly flammable chemicals are used.<br /> * Ensure proper grounding. Be sure to ground metal containers when transferring flammable liquids.<br /> * Keep containers of flammable chemicals closed at all times when not in use to prevent accumulation of flammable vapor concentrations.<br />  <br /> ==Personal Protective Equipment==<br /> * Wear nitrile laboratory gloves, lab coat (flame-resistant lab coats for handling large volumes), safety glasses, clothing that minimizes exposed skin, and close-toed shoes for all work with flammables. If working with flammable liquids that may splash, wear chemical splash goggles. <br /> * If a flammable liquid is also toxic, check glove guide for appropriate gloves and/or minimize glove contact with the liquid. <br /> * Avoid wearing flammable clothing (such as many synthetics) when working with flammable materials.<br /> <br /> Gloving Material Advantages and Disadvantages<br /> BUTYL RUBBER <br /> * Good for ketones and esters<br /> * Poor for gasoline and aliphatic, aromatic, and halogenated hydrocarbons<br /> * Expensive<br /> * Only available as re-useable<br /> * Poor touch sensitivity<br /> NEOPRENE<br /> * Good for acids, bases, alcohols, fuels, peroxides, hydrocarbons, and phenols<br /> * Poor for halogenated and aromatic hydrocarbons<br /> VITON <br /> * Good for chlorinated and aromatic solvents<br /> * Low surface tension repels most liquids<br /> * Good resistance to cuts and abrasions<br /> * Poor for ketones<br /> * Expensive<br /> * Only available as re-useable<br /> * Poor touch sensitivity<br /> VINYL - Polyvinyl Alcohol (PVA) <br /> * Good for aromatic and chlorinated solvents<br /> * Poor for water-based solutions (dissolves in water)<br /> * Avoid: Contact with water or water-based solutions, water solubles<br /> * Only available as re-useable<br /> * Poor touch sensitivity<br /> <br /> ==Transportation and Storage==<br /> * Flammable chemicals should be stored in appropriate areas within the laboratory and away from any potentially incompatible materials.<br /> * Transport flammables in secondary containment, preferably a polyethylene or solvent bottle carrier.<br /> * Suitable fire control devices (such as fire extinguishers) must be available at locations where flammable or combustible liquids are stored. <br /> * Open flames shall not be permitted in flammable liquid storage areas. Flammable and combustible liquids must not be stored in a manner that hinders safe egress.<br /> * If large quantities (&gt;60 gallons) of flammable or combustible liquids are stored, a specially-designed storage room may be required.<br /> * Store these materials in a separate room from water-reactives.<br /> * Flammable and combustible liquids may be stored in a flammable storage cabinet. All such storage cabinets must meet NFPA 30 requirements.<br /> * Flammable liquids shall not be stored in unapproved or residential-type refrigerators. <br /> * Secondary containment is recommended for liquids.<br /> * Avoid storing on the floor.<br /> <br /> ==Waste Disposal==<br /> Because most spent, unused and expired chemicals/materials are considered hazardous wastes, they must be properly disposed of. Do not dispose of chemical wastes by dumping them down a sink, flushing in a toilet or discarding in regular trash containers, unless authorized by OSEH Hazardous Materials Management (HMM). Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information.<br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> <br /> * If skin contact occurs, immediately remove contaminated clothing and rinse with water for at least 15 minutes.<br /> * For eye exposures, immediately rinse eyes with copious amounts of water for at least 15 minutes, while occasionally lifting upper and lower lids, then promptly seek medical attention.<br /> * If large amounts of vapors are inhaled, move person to fresh air immediately and seek medical attention.<br /> * If ingested, seek medical attention immediately.<br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> TREATMENT FACILITIES:<br /> U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> Click here for additional accident and injury reporting information.<br /> <br /> ==Spill Procedure==<br /> When a spill occurs, personal safety should always come first. Alert and clear everyone in the immediate area where the spill occurred. <br /> <br /> A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., less than 1 gallon or 3.5 liters. A major/large chemical spill requires active assistance from emergency personnel.<br /> <br /> Spill Response Steps:<br /> <br /> MINOR CHEMICAL SPILL<br /> * Alert people in immediate area of spill.<br /> * Turn off ignition and heat sources. Don’t light Bunsen burners or turn on other switches.<br /> * Open outside windows, if possible.<br /> * Wear protective equipment, including safety goggles, gloves and long-sleeve lab coat.<br /> * Avoid breathing vapors from spill.<br /> * Confine spill to as small an area as possible.<br /> * Do not wash spill down the drain.<br /> * Use appropriate spill kits/sorbents to neutralize corrosives and/or absorb spill. Collect contaminated materials and residues and place in container and contact OSEH-HMM (734) 763-4568 for proper disposal.<br /> * Clean spill area with water.<br /> <br /> MAJOR CHEMICAL SPILL<br /> * Attend to injured or contaminated persons and remove them from exposure.<br /> * Alert people in the laboratory to evacuate.<br /> * If appropriate turn off ignition and heat sources. Don’t light Bunsen burners or turn on other switches.<br /> * Call University of Michigan Police Department (UMPD) at 911 immediately for assistance.<br /> * Close doors to affected area.<br /> * Post warnings to keep people from entering the area.<br /> * Have person available that has knowledge of incident and laboratory to assist emergency personnel.<br /> <br /> ==Additional Spill Information Links:==<br /> * www.oseh.umich.edu/pdf/chemspil.pdf <br /> * http://www.oseh.umich.edu/emer-chemical.shtml. <br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br />  <br /> ==Training of Personnel==<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> <br /> * [[User:Ccousz|Ccousz]] ([[User talk:Ccousz|talk]]) 17:09, 12 October 2016 (UTC)<br /> *[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:17, 31 October 2016 (UTC)<br /> * [[User:Davebridges|Davebridges]] ([[User talk:Davebridges|talk]]) 14:51, 8 November 2016 (UTC)<br /> *[[User:Iharvey|Iharvey]] ([[User talk:Iharvey|talk]]) 21:18, 10 November 2016 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:11, 5 June 2017 (UTC)<br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ YES X NO<br /> Laboratory Director - Dave Bridges Revision Date - 2016-10-12</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Electrophoresis&diff=1264 2017-06-05T18:10:57Z

<p>Pfeiferl: signed SOP</p> <hr /> <div>[[ Category: SOP ]]<br /> [[ Category: Lab Safety ]]<br /> <br /> ==Description==<br /> This standard operating procedure outlines the process of electrophoresis, including the use of equipment and chemicals necessary for the process. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when performing electrophoresis.<br /> <br /> Electrophoresis uses electrical energy to separate molecules based on their size, structure, and electrical charge.<br /> <br /> ==Potential Hazards==<br /> Electrophoresis equipment can pose significant electrical hazard in the laboratory. Typical electrophoresis units operating at 100 volts can provide a lethal shock of 25 milliamps. Take the following precautions:<br /> <br /> '''Power Supplies:'''<br /> *Inspect to ensure all switches and indicators are in proper working condition and that power cords and leads are undamaged and properly insulated.<br /> *Label equipment with warning: “Danger Electrical Hazard.”<br /> *Connect to ground fault circuit interrupters (GFCIs).<br /> *Use 3-prong plugs.<br /> *Use power supplies with safety features that detect no-load, overload, sudden load change, short circuit, arc or ground leak, etc.<br /> <br /> '''Connecting Leads:'''<br /> *Turn off main power supply before connecting or disconnecting electrical leads.<br /> *With dry gloved hands, connect one lead at a time using one hand only.<br /> *Be sure that leads/banana plugs are fully seated.<br /> <br /> '''Using Equipment:'''<br /> *Don’t run equipment unattended.<br /> *Keep equipment clear of unintentional grounding points and conductors (e.g., sinks or other water sources, metal plates, jewelry, aluminum foil, pipes or other electrical/metal equipment).<br /> *Gel chamber must have a lid or cover with safety interlocks to prevent accidental contact with energized electrodes or buffer solutions.<br /> *Gel chamber exterior must be dry with no spilled solutions. Check for leaks.<br /> *Switch off all power and unplug the leads before opening the gel chamber lid or reaching inside the gel chamber. <br /> �<br /> '''Hazardous chemicals'''<br /> Commonly used in conjunction with electrophoresis work include:<br /> *Ethidium bromide – mutagen, irritant<br /> *Acrylamide – carcinogen, neurotoxin, irritant<br /> *Phenol – corrosive, toxic<br /> *Chloroform – suspect carcinogen, toxic<br /> <br /> Always review the Safety Data Sheet prior to working with any hazardous material.<br /> Laboratory personnel may be exposed to thermal hazards posed by liquefied gels.<br /> <br /> '''Ultraviolet''' (UV) light boxes are often used in visualizing ethidium bromide gels and pose potential exposures to UV radiation.<br /> <br /> ==Engineering and Work Practice Controls==<br /> *Read and follow manufacturer’s instructions for electrophoresis equipment.<br /> *Prepare Standard Operating Procedure relevant to health and safety. <br /> *Consult with Lab Director prior to initial use of electrophoresis equipment. Discussion should include special hazards and safety precautions.<br /> *Measure, mix and handle all hazardous powdered chemicals or gel prep mixtures with hazardous components (e.g., acrylamide monomer, ethidium bromide, phenol, ammonium persulfate, and formaldehyde) in a fume hood.<br /> *Purchase pre-made gels or pre-mixed acrylamide and ethidium bromide solutions instead of making your own.<br /> *Consider using ethidium bromide substitutes.<br /> *Exercise caution when using microwave to liquefy gels – don’t use sealed containers, beware of superheated liquids that may froth up unexpectedly. Let hot gel preps cool to 50°-60°C before adding ethidium bromide or pouring into trays. Wear insulated gloves and point the flask opening away from you.<br /> <br /> ==Protective Equipment==<br /> *Wear lab coat with fully extended sleeves, safety glasses or splash goggles if appropriate, nitrile gloves (latex is not effective), pants, and closed-toe shoes.<br /> *Wear appropriate skin and eye protection for UV radiation work.<br /> <br /> ==Waste Disposal==<br /> '''Hazardous Waste Management:''' Handle and store hazardous waste following the guidelines above for work practice controls, transportation and storage. Contact OSEH Hazardous Materials Management (HMM) at 734-763-4568 with questions and to schedule a pickup of hazardous waste. For more information regarding chemical waste, refer to the HMM section on OSEH’s website at http://www.oseh.umich.edu/hazmats/chemical.shtml. <br /> <br /> '''Non-Hazardous Waste Management:''' Some gels may be considered non-hazardous but still present a health hazard if untreated or discarded into the normal trash. Contact HMM for proper collection and disposal.<br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> <br /> For an actual chemical exposure/injury:<br /> *Remove contaminated clothing. Flush exposed eyes or skin with water for at least 15 minutes. Seek medical attention (see below).<br /> *For inhalation exposure, remove all persons from the contaminated area. Get medical aid. <br /> *If an ambulance is needed, call the University of Michigan Division of Public Safety and Security (DPSS) at 911 to request assistance.<br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form.<br /> <br /> ==Treatment Facilities:==<br /> *U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> *University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> *UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> <br /> Click here for more information.<br /> <br /> ==Spill Procedure==<br /> *When a spill occurs, personal safety should always come first.<br /> *Alert and clear everyone in the immediate area where the spill occurred. <br /> *Follow spill procedures described in the SOP and SDS for the particular chemical spilled.<br /> <br /> '''Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> '''<br /> <br /> <br /> ==Training of Personnel==<br /> <br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when performing electrophoresis.<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> <br /> *[[User:Reddj|Reddj]] ([[User talk:Reddj|talk]]) 15:00, 13 October 2016 (UTC)<br /> *[[User:Iharvey|Iharvey]] ([[User talk:Iharvey|talk]]) 21:19, 10 November 2016 (UTC)<br /> *[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:15, 31 October 2016 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:10, 5 June 2017 (UTC)<br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ YES ☒ NO<br /> <br /> <br /> <br /> Laboratory Director - Dave Bridges Revision Date - 2016-10-13</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Chloroform&diff=1263 2017-06-05T18:10:21Z

<p>Pfeiferl: signed SOP</p> <hr /> <div>[[ Category: SOP ]] <br /> <br /> ==Description==<br /> This standard operating procedure outlines the handling and use of chloroform. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when handling chloroform.<br /> <br /> Chloroform (CAS # = 67-66-3) is a clear, colorless liquid with a pleasant, nonirritating odor that can be detected as low as 11.7 ppm (odor threshold) but more likely detected at levels of 85 to 307 ppm (well above safe exposure limits). It will burn only when it reaches very high temperatures. <br /> <br /> NOTE: Stabilizers are often added to prevent oxidation by air and light, and to prevent chloroform from becoming acidic and corrosive.<br /> <br /> Synonyms include: Trichloromethane, Methane trichloride, Formyl trichloride, Trichloroform, Methenyl trichloride and Freon 20.<br /> <br /> Chloroform is used as an extractant solvent in manufacture of rubber, essential oils, sterols and alkaloids, guttapercha, resins, and in the recovery of fat from waste products; in chemical analysis and assays; and in photographic processing.<br /> It is also used as a general solvent of lacquers, plastics, dyes, fats, greases, gums, oils, adhesives, and waxes, and in the rubber cleaning and dry cleaning industries.<br /> <br /> ==Useful Chloroform Links:==<br /> * http://www.atsdr.cdc.gov/substances/toxsubstance.asp?toxid=16<br /> * http://www.atsdr.cdc.gov/toxfaqs/tf.asp?id=52&amp;tid=16<br /> * http://www.nap.edu/openbook.php?record_id=4911&amp;page=282<br /> <br /> ==Potential Hazards==<br /> * Chloroform has been classified as a potential to probable human carcinogen, based on adequate evidence for carcinogenicity in animals. Some animal studies also show evidence of reproductive and developmental toxicity from chloroform exposure. <br /> * Inhalation of vapors can cause headaches, drowsiness, dizziness, and nausea. At high concentrations disorientation, anesthetic effects, and unconsciousness can occur, but acute toxicity is low.<br /> * Chloroform is an eye, skin and upper respiratory tract irritant.<br /> * Chloroform is not combustible but exposure to fire or high temperatures may lead to formation of phosgene, hydrogen chloride and chlorine, all highly toxic gases.<br /> * Consult the SDS for Chloroform for additional guidance and information.<br /> <br /> Occupational Exposure Limits (OELs):<br /> * MIOSHA: 2 ppm, 8-hour PEL<br /> <br /> <br /> ==Engineering Controls==<br /> Work with chloroform in a chemical fume hood. Dilute solutions (&lt; 10%) may be used on the benchtop in small quantities (&lt; 500 mL).<br /> <br /> ==Work Practice Controls==<br /> * Keep containers of chloroform closed as much as possible.<br /> * Be aware of skin absorption as a possible route of exposure. Plan work so that minimal glove contact is expected, and purchase appropriate gloves for cleaning up small spills. (Refer to the PPE section below, for glove recommendations.)<br /> * Use in the smallest practical quantities for the experiment being performed.<br /> * Do not mix or store with acids; may form toxic gas.<br /> * Thoroughly wash hands when finished handling.<br /> <br /> ==Personal Protective Equipment (PPE)==<br /> * The minimum PPE for work with chloroform is Viton or PVA (Polyvinyl Acetate) laboratory gloves, lab coat, and safety glasses (ANSI/ISEA Z87.1 approved). Keep in mind that chloroform will penetrate nitrile gloves in less than 2.5 minutes.<br /> * If a splash may occur, wear chemical splash goggles and/or a face shield.<br /> * OSEH’s Glove Compatibility webpage can also be used to determine the recommended gloves.<br /> <br /> ==Transportation and Storage==<br /> * Do not store chloroform with incompatibles. Chloroform is not compatible with the following: acetone, strong bases, alkalis, chemically-active metals (such as aluminum, magnesium, sodium, or potassium), dinitrogen tetroxide, fluorine, disilane, sodium methylate, triisopropylphosphine, and solid potassium tert-butoxide.<br /> * Transport chloroform in secondary containment, preferably a polyethylene or other non-reactive acid/solvent bottle carrier.<br /> * Store in secondary containment.<br /> * Avoid storing on the floor.<br /> * Refer to OSEH’s Chemical Storage webpage for additional information on the storage of hazardous materials.<br /> <br /> ==Waste Disposal==<br /> Because most spent, unused and expired chemicals/materials are considered hazardous wastes, they must be properly disposed of. Do not dispose of chemical wastes by dumping them down a sink, flushing in a toilet or discarding in regular trash containers, unless authorized by OSEH Hazardous Materials Management (HMM). Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information.<br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> * Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical aid.<br /> * Skin: In case of contact, immediately flush skin with plenty of water. Remove contaminated clothing and shoes. Get medical aid. Wash clothing before reuse.<br /> * Ingestion: Get medical aid immediately. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If vomiting occurs naturally, have victim lean forward.<br /> * Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. <br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> ===TREATMENT FACILITIES:===<br /> U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> Click here for more information.<br /> <br /> ==Spill Procedure==<br /> * When a spill occurs, personal safety should always come first.<br /> * Alert and clear everyone in the immediate area where the spill occurred. <br /> * Use proper personal protective equipment (PPE) as noted above.<br /> <br /> A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., (less than 1 Gallon or 3.5 Liters). A major/large chemical spill requires active assistance from emergency personnel.<br /> <br /> ===Spill Response Steps:===<br /> <br /> ===MINOR CHEMICAL SPILL===<br /> * Alert people in immediate area of spill.<br /> * Open outside windows, if possible.<br /> * Use proper personal protective equipment as indicated above.<br /> * Avoid breathing vapors from spill.<br /> * Confine spill to as small an area as possible.<br /> * Do not wash spill down the drain.<br /> * Use appropriate spill kits/sorbents to absorb spill. Collect contaminated materials and residues and place in container. Contact OSEH-HMM (734) 763-4568 for proper disposal.<br /> * Clean spill area with water.<br /> <br /> ===MAJOR CHEMICAL SPILL===<br /> Report large chemical spills greater than 1 Gallon or 3.5 Liters in corridors or common areas, e.g., hallways, elevators, eating areas, rest rooms, offices, etc., to U-M Division of Public Safety and Security (DPSS) by calling 911.<br /> * Attend to injured or contaminated persons and remove them from exposure.<br /> * Alert people in the laboratory to evacuate.<br /> * Call U-M Division of Public Safety and Security (DPSS) at 911 immediately for assistance.<br /> * Close doors to affected area.<br /> * Post warnings to keep people from entering the area.<br /> * Have person available that has knowledge of incident and laboratory to assist emergency personnel.<br /> <br /> Additional Spill Links:<br /> * www.oseh.umich.edu/pdf/chemspil.pdf <br /> * http://www.oseh.umich.edu/emer-chemical.shtml<br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> ==Training of Personnel==<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling chloroform.<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> <br /> * [[User:Davebridges|Davebridges]] ([[User talk:Davebridges|talk]]) 14:42, 15 August 2016 (UTC)<br /> *[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:15, 31 October 2016 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:09, 5 June 2017 (UTC)<br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ YES X NO<br /> <br /> <br /> <br /> Laboratory Director - Dave Bridges Revision Date - 2016-08-15</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Chloroform&diff=1262 2017-06-05T18:09:56Z

<p>Pfeiferl: signed SOP</p> <hr /> <div>[[ Category: SOP ]] <br /> <br /> ==Description==<br /> This standard operating procedure outlines the handling and use of chloroform. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls, personal protective equipment, and disposal techniques when handling chloroform.<br /> <br /> Chloroform (CAS # = 67-66-3) is a clear, colorless liquid with a pleasant, nonirritating odor that can be detected as low as 11.7 ppm (odor threshold) but more likely detected at levels of 85 to 307 ppm (well above safe exposure limits). It will burn only when it reaches very high temperatures. <br /> <br /> NOTE: Stabilizers are often added to prevent oxidation by air and light, and to prevent chloroform from becoming acidic and corrosive.<br /> <br /> Synonyms include: Trichloromethane, Methane trichloride, Formyl trichloride, Trichloroform, Methenyl trichloride and Freon 20.<br /> <br /> Chloroform is used as an extractant solvent in manufacture of rubber, essential oils, sterols and alkaloids, guttapercha, resins, and in the recovery of fat from waste products; in chemical analysis and assays; and in photographic processing.<br /> It is also used as a general solvent of lacquers, plastics, dyes, fats, greases, gums, oils, adhesives, and waxes, and in the rubber cleaning and dry cleaning industries.<br /> <br /> ==Useful Chloroform Links:==<br /> * http://www.atsdr.cdc.gov/substances/toxsubstance.asp?toxid=16<br /> * http://www.atsdr.cdc.gov/toxfaqs/tf.asp?id=52&amp;tid=16<br /> * http://www.nap.edu/openbook.php?record_id=4911&amp;page=282<br /> <br /> ==Potential Hazards==<br /> * Chloroform has been classified as a potential to probable human carcinogen, based on adequate evidence for carcinogenicity in animals. Some animal studies also show evidence of reproductive and developmental toxicity from chloroform exposure. <br /> * Inhalation of vapors can cause headaches, drowsiness, dizziness, and nausea. At high concentrations disorientation, anesthetic effects, and unconsciousness can occur, but acute toxicity is low.<br /> * Chloroform is an eye, skin and upper respiratory tract irritant.<br /> * Chloroform is not combustible but exposure to fire or high temperatures may lead to formation of phosgene, hydrogen chloride and chlorine, all highly toxic gases.<br /> * Consult the SDS for Chloroform for additional guidance and information.<br /> <br /> Occupational Exposure Limits (OELs):<br /> * MIOSHA: 2 ppm, 8-hour PEL<br /> <br /> <br /> ==Engineering Controls==<br /> Work with chloroform in a chemical fume hood. Dilute solutions (&lt; 10%) may be used on the benchtop in small quantities (&lt; 500 mL).<br /> <br /> ==Work Practice Controls==<br /> * Keep containers of chloroform closed as much as possible.<br /> * Be aware of skin absorption as a possible route of exposure. Plan work so that minimal glove contact is expected, and purchase appropriate gloves for cleaning up small spills. (Refer to the PPE section below, for glove recommendations.)<br /> * Use in the smallest practical quantities for the experiment being performed.<br /> * Do not mix or store with acids; may form toxic gas.<br /> * Thoroughly wash hands when finished handling.<br /> <br /> ==Personal Protective Equipment (PPE)==<br /> * The minimum PPE for work with chloroform is Viton or PVA (Polyvinyl Acetate) laboratory gloves, lab coat, and safety glasses (ANSI/ISEA Z87.1 approved). Keep in mind that chloroform will penetrate nitrile gloves in less than 2.5 minutes.<br /> * If a splash may occur, wear chemical splash goggles and/or a face shield.<br /> * OSEH’s Glove Compatibility webpage can also be used to determine the recommended gloves.<br /> <br /> ==Transportation and Storage==<br /> * Do not store chloroform with incompatibles. Chloroform is not compatible with the following: acetone, strong bases, alkalis, chemically-active metals (such as aluminum, magnesium, sodium, or potassium), dinitrogen tetroxide, fluorine, disilane, sodium methylate, triisopropylphosphine, and solid potassium tert-butoxide.<br /> * Transport chloroform in secondary containment, preferably a polyethylene or other non-reactive acid/solvent bottle carrier.<br /> * Store in secondary containment.<br /> * Avoid storing on the floor.<br /> * Refer to OSEH’s Chemical Storage webpage for additional information on the storage of hazardous materials.<br /> <br /> ==Waste Disposal==<br /> Because most spent, unused and expired chemicals/materials are considered hazardous wastes, they must be properly disposed of. Do not dispose of chemical wastes by dumping them down a sink, flushing in a toilet or discarding in regular trash containers, unless authorized by OSEH Hazardous Materials Management (HMM). Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information.<br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> * Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical aid.<br /> * Skin: In case of contact, immediately flush skin with plenty of water. Remove contaminated clothing and shoes. Get medical aid. Wash clothing before reuse.<br /> * Ingestion: Get medical aid immediately. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If vomiting occurs naturally, have victim lean forward.<br /> * Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. <br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> ===TREATMENT FACILITIES:===<br /> U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> Click here for more information.<br /> <br /> ==Spill Procedure==<br /> * When a spill occurs, personal safety should always come first.<br /> * Alert and clear everyone in the immediate area where the spill occurred. <br /> * Use proper personal protective equipment (PPE) as noted above.<br /> <br /> A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., (less than 1 Gallon or 3.5 Liters). A major/large chemical spill requires active assistance from emergency personnel.<br /> <br /> ===Spill Response Steps:===<br /> <br /> ===MINOR CHEMICAL SPILL===<br /> * Alert people in immediate area of spill.<br /> * Open outside windows, if possible.<br /> * Use proper personal protective equipment as indicated above.<br /> * Avoid breathing vapors from spill.<br /> * Confine spill to as small an area as possible.<br /> * Do not wash spill down the drain.<br /> * Use appropriate spill kits/sorbents to absorb spill. Collect contaminated materials and residues and place in container. Contact OSEH-HMM (734) 763-4568 for proper disposal.<br /> * Clean spill area with water.<br /> <br /> ===MAJOR CHEMICAL SPILL===<br /> Report large chemical spills greater than 1 Gallon or 3.5 Liters in corridors or common areas, e.g., hallways, elevators, eating areas, rest rooms, offices, etc., to U-M Division of Public Safety and Security (DPSS) by calling 911.<br /> * Attend to injured or contaminated persons and remove them from exposure.<br /> * Alert people in the laboratory to evacuate.<br /> * Call U-M Division of Public Safety and Security (DPSS) at 911 immediately for assistance.<br /> * Close doors to affected area.<br /> * Post warnings to keep people from entering the area.<br /> * Have person available that has knowledge of incident and laboratory to assist emergency personnel.<br /> <br /> Additional Spill Links:<br /> * www.oseh.umich.edu/pdf/chemspil.pdf <br /> * http://www.oseh.umich.edu/emer-chemical.shtml<br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> ==Training of Personnel==<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling chloroform.<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> <br /> * [[User:Davebridges|Davebridges]] ([[User talk:Davebridges|talk]]) 14:42, 15 August 2016 (UTC)<br /> <br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ YES X NO<br /> <br /> *[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:15, 31 October 2016 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:09, 5 June 2017 (UTC)<br /> <br /> Laboratory Director - Dave Bridges Revision Date - 2016-08-15</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Centrifuge_Safety&diff=1261 2017-06-05T18:09:25Z

<p>Pfeiferl: signed SOP</p> <hr /> <div>[[ Category: SOP ]]<br /> [[ Category: Lab Safety ]]<br /> <br /> ==Description==<br /> This standard operating procedure outlines the use of centrifuges. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls and personal protective equipment when using centrifuges.<br /> <br /> Centrifuges are machines used to separate solids from liquids in a suspension. The spinning motion of a centrifuge produces centrifugal forces that separate substances of greater and lesser densities. <br /> <br /> ==Potential Hazards==<br /> The centrifuge must be loaded carefully balanced. An unbalanced load may present a risk to both the machine and to persons nearby. Centrifuge rotors should never be touched while the rotor is spinning, as they present a significant personal risk when in motion. Another potential hazard is the possible aerosolization of harmful samples during centrifuge operation.<br /> <br /> ==Engineering Controls==<br /> If centrifuging hazardous materials, care should be taken to use tightly capped tubes and/or sealable safety cups or rotors that can be loaded and unloaded in a fume hood or biosafety cabinet, depending on the hazard. <br /> <br /> ==Work Practice Controls==<br /> The following information may be integrated into a lab-specific standard operating procedure (SOP) for centrifuge use. <br /> <br /> 1. ''Preventive Maintenance''<br /> <br /> A. Establish preventive maintenance schedule: Including regular cleaning of centrifuge interior to prevent damage and avoid costly repairs. Reference centrifuge operator’s manual or contact manufacturer for guidance. <br /> <br /> Equipment repair and adjustments shall only be conducted by qualified service technicians.<br /> <br /> B. Maintain log book: For all high speed centrifuges and ultracentrifuges include run dates, durations, speeds, total rotor revolutions, and notes on rotor condition.<br /> <br /> Retire rotors after manufacturer’s recommended life span except where annual stress test demonstrates absence of structural flaws. Note: Rotor life span may be reduced or warranty voided if autoclaved; contact manufacturer for guidance.<br /> <br /> <br /> 2. ''Planning for Use''<br /> <br /> A. Complete lab-specific training for the centrifuge.<br /> <br /> B. Wear appropriate PPE: Including safety eyewear, gloves, lab coat, and appropriate street clothing (i.e., long pants and closed-toe shoes). Ensure gloves are compatible with hazard(s).<br /> <br /> C. Inspect centrifuge:<br /> * Ensure tubes are rated for intended use (speed, temperature, and chemical resistance).<br /> * Ensure rotor is compatible with centrifuge and seated on the drive correctly.<br /> * Ensure rotor and safety cups/buckets are free of cracks and deformities.<br /> * Ensure rotor O-ring is not cracked, missing, or worn.<br /> * Ensure safety cups/buckets are attached correctly and able to move freely.<br /> <br /> Contact a qualified service technician if inspection identifies centrifuge components requiring repair or replacement.<br /> <br /> D. Prepare centrifuge tubes for loading:<br /> * Inspect centrifuge tubes before use.<br /> * Follow manufacturer’s filling limits for tubes. Do not overfill or underfill tubes.<br /> * For biohazardous materials, disinfect outside of tubes prior to removal from biosafety cabinet and loading into rotor.<br /> <br /> When centrifuging hazardous materials, use tightly capped tubes and/or sealable safety cups or rotors that can be loaded and unloaded in a fume hood or biosafety cabinet, depending on hazard. If a specific procedure does not allow for this, contact Occupational Safety and Environmental Health (OSEH) at (734) 647-1143 for guidance.<br /> <br /> E. Use in-line filter: For high speed centrifuges and ultracentrifuges, use in-line filters to prevent contamination of vacuum pump and pump oil. Provide secondary containment for vacuum pump.<br /> <br /> <br /> 3. ''Centrifuge Operation''<br /> <br /> A. Balance centrifuge: Follow manufacturer’s instructions for proper centrifuge balancing steps.<br /> <br /> B. Start run<br /> * Do not leave centrifuge until full operating speed is reached and it appears to be running safely without incident.<br /> * Stop centrifuge immediately if you notice any unusual noises or shaking. Confirm rotor is balanced.<br /> <br /> To prevent rotor failure, do not exceed maximum speed and maximum mass limits for the rotor. You must reduce rotor speed if sample density calculations indicate maximum mass limits will be exceeded; contact manufacturer for guidance.<br /> <br /> <br /> 4 ''Sample Removal''<br /> <br /> A. Stop run: Ensure centrifuge comes to complete stop before opening cover. When centrifuging hazardous materials, wait at least 10 minutes after run to allow aerosols to settle before opening centrifuge.<br /> <br /> B. Check for leaks/spills: In samples, rotor, safety cups/buckets, and centrifuge well.<br /> <br /> C. Open sealable tubes/safety cups/rotors: Wear appropriate PPE and open inside fume hood or biosafety cabinet, depending on hazard.<br /> <br /> ==Personal Protective Equipment==<br /> When operating the centrifuge, proper eye protection (glasses/goggles), gloves, lab coat, and close-toe footwear should be worn. Gloves should be appropriate for the present hazard.<br /> <br /> ==Waste Disposal==<br /> Centrifuge Disposal<br /> <br /> 1. For biohazardous materials, clean/disinfect centrifuge and remove/cross out biohazard sticker. Attach note on centrifuge describing what has been done.<br /> <br /> 2. For radioactive materials, request radiation safety survey and signage before disposal of centrifuge.<br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> If the employee is in need of emergency medical attention, call 911 immediately. <br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> TREATMENT FACILITIES:<br /> U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> Click here for more information.<br /> <br /> ==Spill Procedure==<br /> 1. Mechanical Failure<br /> <br /> Turn off centrifuge immediately and unplug power cord. Do not use centrifuge again until inspected by qualified service technician.<br /> <br /> 2. Hazardous Material Spill/Exposure<br /> <br /> A. Turn off centrifuge immediately. Keep centrifuge cover closed for at least 30 minutes to reduce aerosolization of hazardous material.<br /> <br /> B. Follow spill, exposure, and incident reporting instructions.<br /> * For chemical, radioactive, and biohazard spills, contact OSEH at (734) 647-1143 for assistance and guidance.<br /> <br /> Remember:<br /> * Conduct rotor or safety cup/bucket cleanup in nearest biosafety cabinet or fume hood, depending on hazard.<br /> * Use tongs or forceps to avoid contact with sharp-edged debris.<br /> * Avoid alkaline cleaners for aluminum centrifuge components.<br /> * Avoid abrasive wire brushes for cleaning.<br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> ==Training of Personnel==<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling centrifuges.<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> <br /> * [[User:Ccousz|Ccousz]] ([[User talk:Ccousz|talk]]) 17:19, 14 October 2016 (UTC)<br /> *[[User:Iharvey|Iharvey]] ([[User talk:Iharvey|talk]]) 21:18, 10 November 2016 (UTC)<br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:09, 5 June 2017 (UTC)<br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ YES ☒ NO<br /> <br /> Laboratory Director - Dave Bridges Revision Date - 2016-10-14</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Animal_Anesthetics&diff=1260 2017-06-05T18:09:00Z

<p>Pfeiferl: signed SOP</p> <hr /> <div>[[ Category: SOP ]]<br /> [[ Category: Lab Safety]]<br /> <br /> ==Description==<br /> This standard operating procedure outlines the handling and use of animal anesthetics including: isoflurane, halothane, enflurane and ether. Review this document and supply the information required in order to make it specific to your laboratory. In accordance with this document, laboratories should use appropriate controls and personal protective equipment when handling animal anesthetics.<br /> <br /> ==Procedure Location==<br /> The use of animal anesthetics must be performed in an area with good ventilation and controls to capture and exhaust waste anesthetic gases.<br /> <br /> ==Potential Hazards==<br /> Anesthetic gas and vapor that leaks during medical or research procedures are considered waste anesthetic gases (WAGs). University faculty, staff and students should be aware of the potential risks of WAGs and be advised to take appropriate precautions to reduce exposures. Workers acutely exposed to excessive amounts of anesthetic gas can experience symptoms of drowsiness, headache, nausea, poor judgment and loss of coordination. Chronic symptoms of over-exposure can include liver, kidney and reproductive effects.  Safety precautions include the use of an approved gas scavenging system, or using the agent inside a certified chemical fume hood.<br /> <br /> The use of ether is not recommended because it is flammable and a mutagen. Be certain that there are no ignition sources present when handling ether. There are restrictions concerning the use of ether with animals. Contact OSEH at (734) 647-1143 concerning the use of ether.<br /> <br /> ==Engineering Controls==<br /> Anesthetics should not be handled on the bench top without special ventilation or a scavenging system. Anesthetic gas filtering cartridges, snorkel exhaust, fume hoods or other scavenging systems must be used. ULAM provides ventilated procedure rooms designed for use of anesthetic gases in many areas. Fume hoods provide the best protection against exposure to anesthetics in the laboratory and are the preferred ventilation control device when handling greater than 100 cc outside of the original container. Always handle large quantities of ethyl ether in a fume hood due to the flammable nature of the material. If your research does not permit the handling of large quantities of ethyl ether in your fume hood, contact OSEH to review the adequacy of all special ventilation.<br /> <br /> Liquid anesthetics administered with a vaporizer must be scavenged. When used properly, vaporizers equipped with activated charcoal canisters (e.g. F/Air) are effective in removing halogenated waste gases. The F/Air canister containing activated charcoal will absorb waste anesthetics for about 12 hours.  Note: F/Air Canisters only absorb halogenated anesthetics (e.g. isoflurane, halothane).  Immediately before using any anesthesia machine, the F/Air canister should be removed and weighed to evaluate the remaining absorption capacity.  The weight should be recorded and dated on the side of the canister. Immediately following the use of an anesthesia machine, the number of hours the machine was in use should be recorded next to the dated weight information.<br />       <br /> Canisters that exceed 12 hours of use or 50 grams of accumulated weight (whichever comes first) must be removed and placed in a sealed plastic bag and disposed of as a hazardous waste through OSEH Hazardous Materials Management (HMM) at (734) 763-4568. Thoroughly clean the induction chamber immediately after each use to avoid residual anesthetic waste release into the environment (which can continue to be released for up to three hours).  Please refer to the OSEH Guideline entitled Anesthetic Gas Use for additional information on the safe use of anesthetic gases.<br /> <br /> ==Work Practice Controls==<br /> All anesthetic agents must be clearly labeled with the correct chemical name. Handwritten labels are acceptable; chemical formulas and structural formulas are not acceptable.<br /> <br /> Always keep the flow rate of anesthetics to the animal as low as possible during the procedure. High flow rates can increase your exposure to the anesthetic. It is also important to move the point of potential gas release as close to the exhaust system as possible to increase capture of the chemical.<br /> <br /> Do not permit containers to remain open on the bench top. The odor thresholds for most liquid anesthetics (except for ether) are well above permissible exposure limits. If you smell the anesthetic the control procedures you are using are inadequate and must be re-evaluated.<br /> <br /> ==Personal Protective Equipment (PPE)==<br /> Eye protection in the form of safety glasses must be worn at all times when handling anesthetic agents. Ordinary (street) prescription glasses do not provide adequate protection<br /> <br /> Single use nitrile or latex gloves must be worn when handling anesthetic agents as well as lab coats, closed toed shoes and pants. Additional protective clothing should be worn if the possibility of skin contact is likely.<br /> <br /> ==Transportation and Storage==<br /> Ethers form potentially explosive peroxides after exposure to air and light. Since these chemicals are packaged in an air atmosphere, peroxides can form even though the containers have not been opened. Write the date received and date opened on all containers of ether. Opened containers of ether should be discarded within 12 months of opening. Even closed containers of ether must be discarded by the expiration date through OSEH-HMM (734) 763-4568.<br /> <br /> Halogenated liquid anesthetic agents (i.e. halothane, enflurane, isoflurane) are not flammable but do have limited shelf life. Be certain to date the chemical when it is opened and to check expiration date before use.<br /> <br /> Always purchase the smallest quantity required for use. Ether used for anesthetic purposes should be purchased in the smallest quantity available (typically 150 cc, Fisher Scientific E136-150) due to its short (12 month) shelf life after it is opened.<br /> <br /> ==Waste Disposal==<br /> Anesthetic agents are hazardous wastes. Contact OSEH-HMM at (734) 763-4568 for waste containers, labels, manifests, waste collection and for any questions regarding proper waste disposal. Also refer to OSEH’s Hazardous Waste webpage for more information.<br /> <br /> ==Exposures/Unintended Contact==<br /> <br /> '''If the employee is in need of emergency medical attention, call 911 immediately.''' <br /> <br /> Wash hands and arms with soap and water immediately following any skin contact with anesthetic agents. Flush eyes for 15 minutes following eye contact.<br /> <br /> Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. <br /> <br /> Report all work related accidents, injuries, illnesses or exposures to WorkConnections within 24 hours by completing and submitting the Illness and Injury Report Form. Follow the directions on the WorkConnections website Forms Instructions to obtain proper medical treatment and follow-up.<br /> <br /> Complete the OSEH Laboratory Incident and Near-Miss Report form. <br /> <br /> ==Treatment Facilities:==<br /> *U-M Occupational Health Services -- Campus Employees<br /> Mon-Fri 7:30 am - 4:30 pm<br /> After hours - go to UM Hospital Emergency Dept. – Urgent Care Clinic<br /> C380 Med Inn building<br /> 1500 East Medical Center Drive, Ann Arbor (734) 764-8021<br /> *University Health Services -- University students (non-life threatening conditions)<br /> Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm<br /> Contact for current hours as they may vary<br /> 207 Fletcher Street, Ann Arbor (734) 764-8320<br /> *UMHS Emergency Department -- after clinic hours or on weekends<br /> 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666<br /> <br /> Click here for additional accident and injury reporting information.<br /> <br /> ==Spill Procedure==<br /> Ether is extremely flammable. If ether is spilled immediately assess and deactivate potential ignition sources. Be prepared for a potential fire and ensure your safety and others first.<br /> <br /> Anticipate spills by having the appropriate clean up equipment on hand. Spill materials for anesthetic agents are designed to control the liquid portion of the spill and minimize the production of vapors. Never use paper towels on large spills of anesthetic agents because it exacerbates vapor production.<br /> <br /> *When a spill occurs, personal safety should always come first.<br /> *Alert and clear everyone in the immediate area where the spill occurred. <br /> <br /> A minor (small) chemical spill is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel, i.e., less than 1 liter. A major/large chemical spill requires active assistance from emergency personnel.<br /> <br /> ==Additional Spill Response Steps:==<br /> <br /> ===MINOR CHEMICAL SPILL===<br /> *Alert people in immediate area of spill.<br /> *If spilled material is flammable, turn off ignition and heat sources. Don’t light Bunsen burners or turn on other switches.<br /> *Open outside windows, if possible.<br /> *Wear protective equipment, including safety goggles, gloves and long-sleeve lab coat.<br /> *Avoid breathing vapors from spill.<br /> *Confine spill to as small an area as possible.<br /> *Do not wash spill down the drain.<br /> *Use appropriate spill kits/sorbents to absorb spill. Collect contaminated materials and residues and place in container. Contact OSEH-HMM (734) 763-4568 for proper disposal.<br /> *Clean spill area with water.<br /> <br /> ===MAJOR CHEMICAL SPILL===<br /> *Attend to injured or contaminated persons and remove them from exposure.<br /> *Alert people in the laboratory to evacuate.<br /> *If spilled material is flammable, turn off ignition and heat sources. Don’t light Bunsen burners or turn on other switches.<br /> *Call University of Michigan Division of Public Safety and Security (DPSS) at 911 immediately for assistance.<br /> *Close doors to affected area.<br /> *Post warnings to keep people from entering the area.<br /> *Have person available that has knowledge of incident and laboratory to assist emergency personnel.<br /> <br /> ==Additional Spill Links:==<br /> • www.oseh.umich.edu/pdf/chemspil.pdf <br /> • http://www.oseh.umich.edu/emer-chemical.shtml. <br /> <br /> Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. Register with the University of Michigan Emergency Alert System via Wolverine Access.<br /> <br /> ==Training of Personnel==<br /> All personnel are required to complete the General Laboratory Safety Training session (BLS025w or equivalent) via OSEH’s My LINC website. Furthermore, all personnel shall read and fully adhere to this SOP when handling animal anesthetics.<br /> <br /> ==Certification==<br /> I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing &lt;nowiki&gt;* ~~~~&lt;/nowiki&gt; in the list below:<br /> <br /> *[[User:Reddj|Reddj]] ([[User talk:Reddj|talk]]) 14:44, 13 October 2016 (UTC)<br /> <br /> * [[User:Iharvey|Iharvey]] ([[User talk:Iharvey|talk]]) 19:34, 10 November 2016 (UTC)<br /> <br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:09, 5 June 2017 (UTC)<br /> Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ YES X NO<br /> <br /> Laboratory Director - Dave Bridges Revision Date - 2016-10-13</div>

Pfeiferl https://bridgeslab.sph.umich.edu/protocols/index.php?title=Safety_and_Animal_Training&diff=1259 2017-06-05T18:08:11Z

<p>Pfeiferl: signed SOP</p> <hr /> <div>These are the classes that lab staff need to take at Michigan:<br /> <br /> ==General Lab Safety==<br /> These are offered through MyLink at https://maislinc.umich.edu/. All lab personnel need to take these<br /> <br /> * BLS025w ONLINE REQUIRED General Laboratory Safety Training (online)<br /> * BLS023 REQUIRED Centrifuge Training (classroom)<br /> * BLS013w ONLINE REQUIRED Autoclave Standard Operating Procedures (online)<br /> <br /> If you will be working with radioactive materials, you will have to take this course as well:<br /> <br /> * RSS006 REQUIRED Radiation Safety Orientation (classroom)<br /> <br /> If you will be working with any viruses you will have to take this course as well:<br /> <br /> * BLS008 REQUIRED Working Safely with Viral Vectors (classroom)<br /> <br /> If you plan to breed animals, you can also take this course:<br /> <br /> *ULAM-10125 Breeding Colony Management for Rats and Mice - Recommended (online)<br /> <br /> ==Animals==<br /> If you will be working with animals you will have to take these classes. They are all offered through ULAM MLearning (https://trainingportal.med.umich.edu/). This requires a levels 2 password.<br /> <br /> * ULAM-10000 Orientation (online)<br /> * ULAM-10050 Hazards pt.1 (online)<br /> * ULAM-10055 Hazards pt.2 (classroom)<br /> * ULAM-10100 Intro Mouse/Rat (online)<br /> * ULAM-10131 Animal Room Procedures pt.1 (online)<br /> * ULAM-10132 Animal Room Procedures pt.2 (classroom)<br /> * ULAM-10105 Lab Mouse Workshop (classroom)<br /> <br /> ==Lab Standard Operating Procedures==<br /> For lab specific safety training please review the SOP's and sign them if you will be using any of them. All lab SOP's are available [[:Category: SOP|here]]<br /> <br /> *[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:08, 5 June 2017 (UTC)<br /> [[ Category: Lab Safety ]]<br /> [[ Category: Training ]]<br /> [[Category: SOP]]</div>

Pfeiferl