https://bridgeslab.sph.umich.edu/protocols/api.php?action=feedcontributions&feedformat=atom&user=Nicwhite 2026-04-19T18:20:13Z User contributions MediaWiki 1.45.1 https://bridgeslab.sph.umich.edu/protocols/index.php?title=Rapamycin_Injections&diff=268 2009-07-28T15:10:02Z

<p>Nicwhite: /* Preparation */</p> <hr /> <div>==Materials==<br /> *Tween 80 - Sigma P1754<br /> *PEG - Sigma P3265<br /> <br /> ==Preparation==<br /> *Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.<br /> *Dissolve rapamycin at 20 mg/mL in water.<br /> *Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).</div>

Nicwhite