Triglyceride Assay from Drosophila (German Method)
Protocol
- Use 5 female or 8 Male flies for Assay
- Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock)
- Heat samples in 70C waterbath for 5 minutes
- Spin samples @ 5000G for 1 minute
- Transfer 500ul of supernatent in new microfuge tube
- Spin @ 14000G for 3 minutes
- Add 50ul of sample to 200ul of TG solution in a 96 well plate
- Incubate plate @ 37C for 5 mins
- Measure 540nm absorbance