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TaqMan SNP Assay

127 bytes removed, 16:39, 16 November 2015
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== Protocol ==
# Dilute gDNA samples to ng20ng/ulapprox.
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).# Add Reaction Mix (5.25 ul) added to each well (Table 1.)# Diluted gDNA samples each added to a single well (Table 1.)
'''Table 1. Reaction Mix for full Full 96 well plate'''
{| class="wikitable"
|-
|26.4 ul
|-
!!5.25 ul!554.4 ul|}   # Reaction Mix gDNA Sample (5.25 20ng/ul) added to each well (see Table 2.)# Diluted gDNA samples each added to a single well (see Table 2.)    '''Table 2. for full 96 well plate'''{| class="wikitable"|-! Component! 1X volume! 96X Volume (+ 10% excess)|-|Reaction Master Mix|5.25 ul |554.4 ul|-|gDNA samples|4.75 50 ul
|501.6 ul
|-
Lmcallan
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