42
edits
Changes
no edit summary
== Protocol ==
# Dilute gDNA samples to 1-5 ng20ng/ulapprox.
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).# Add Reaction Mix (5.25 ul) added to each well (Table 1.)# Diluted gDNA samples each added to a single well (Table 1.)
{| class="wikitable"
|-
|0.25 ul
|26.4 ul
|-
|gDNA Sample (20ng/ul)
|4.50 ul
|501.6 ul
|-
!
!5.25 10 ul!554.4 1056 ul|}