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== Protocol ==
# Dilute gDNA samples to 1-5 ng20ng/ulapprox.
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).# Add Reaction Mix (5.25 ul) added to each well (Table 1.)# Diluted gDNA samples each added to a single well (Table 1.)
'''Table 1. Full 96 well plate'''
{| class="wikitable"
|-
|26.4 ul
|-
|gDNA Sample (20ng/ul)|54.25 50 ul|554501.4 6 ul|-!!10 ul!1056 ul|}