Changes

# Dilute gDNA samples to 1-5 ng20ng/ulapprox.# Place gDNA, TaqMan® Genotyping Master Mix(40X), TaqMan® Genotyping Assay (2X) on ice.# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).# Add Reaction Mix (5.25 ul) added to each well (Table 1.)# Diluted gDNA samples each added to a single well (Table 1.)  '''Table 1. Full 96 well plate'''{| class="wikitable"|-! Component! 1X volume! 96X Volume (+ 10% excess)|-|TaqMan® Genotyping Master Mix (2X)|5.00 ul |528.0 ul|-|TaqMan® Genotyping Assay (40X)|0.25 ul|26.4 ul|-|gDNA Sample (20ng/ul)|4.50 ul|501.6 ul|-!!10 ul!1056 ul|}