Changes

*TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).*TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems).*StepOnePlus Real-Time PCR System (Applied Biosystems).*PC/Laptop with StepOnePlus Software installed.*MicroAmp Fast 96-Well Plates.*Optical Plate Covers.*Nuclease-Free Water *Sample Genomic DNA (extracted as described in [[Blood DNA Extraction]])*Postive control(s) (Minor allele) (''optional, but recommended'')

MicroAmp Fast 96-Well Plates== Protocol ==# Dilute gDNA samples to 20ng/ul approx.# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).# Add Reaction Mix (5.25 ul) added to each well (Table 1.)# Diluted gDNA samples each added to a single well (Table 1.)

'''Table 1. Full 96 well plate'''{| class== Protocol =="wikitable"|-! Component! 1X volume! 96X Volume (+ 10% excess)|-|TaqMan® Genotyping Master Mix (2X)|5.00 ul |528.0 ul|-|TaqMan® Genotyping Assay (40X)|0.25 ul|26.4 ul|-|gDNA Sample (20ng/ul)|4.50 ul|501.6 ul|-!!10 ul!1056 ul|}