Changes

Splitting Cells

533 bytes added, 19:20, 17 January 2020

added SOP hyperlinks

==SOP==

*[[SOP-_Biosafety Cabinet|SOP- Biosafety Cabinet]]

*[[SOP-_Cryogenic Materials|SOP- Cryogenic Materials]]

*[[SOP-_Vacuum Pumps|SOP- Vacuum Pumps]]

==Materials==

*Media (FBS or NCS as required): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM

(To prepare DMEM:

1 bottle of DMEM found in 4c in cell culture room

1 tube FBS found in -80 in cell culture room

5 mLs PSG found in door of 4c in cell culture room

500 mL bottle taken from lab

filter found on metal rack behind fume hood

•pour all ingredients into DMEM bottle

•screw filter onto 500 mL bottle

•attach hose onto arm of filter

•pour mixture into filter)

*PBS -/-

*0.05% Trypsin

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