5
edits
Changes
added clarifying notes
* Add 100 uL cholesterol reagent to each well using the multichannel pipet.
* Start a timer
* Add 2 uL serum (mouse sample) to each well* In the middle of the time add the standards(2, 4, 6, 8 uL of 10x and 1, 1.5, 2 uL of the undiluted cholesterol standard)
* The assay takes 15 minutes to develop at room temperature, and is stable for 30 minutes. That means if the timer hits 30 minutes you wont be able to finish in time, so stop and start the plate.
* Once all samples are added, incubate plate at room temperature for 30 minutes