Open main menu

Rapamycin Injections

Revision as of 15:10, 28 July 2009 by Nicwhite (Talk | contribs) (→‎Preparation)

Revision as of 15:10, 28 July 2009 by Nicwhite (Talk | contribs) (→‎Preparation)

Materials

  • Tween 80 - Sigma P1754
  • PEG - Sigma P3265

Preparation

  • Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip.
  • Dissolve rapamycin at 20 mg/mL in water.
  • Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
Last modified on 8 June 2020, at 17:07