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RT-PCR primer design for ChIP

6 bytes added, 17:17, 25 January 2016
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# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to [[Locating ChIP-seq Peaks protocol)from ENCODE]]. Make sure this the genetic sequence is species appropriate. If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
# The genetic region entered for primer search should be around 400 bp.
# Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]
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