RIPA Buffer

Revision as of 20:57, 27 January 2016 by Davebridges (Talk | contribs) (RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol)

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Revision as of 20:57, 27 January 2016 by Davebridges (Talk | contribs) (RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

RIPA Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
Tris pH7.4 50mM 500uL 1M Chemical Shelf
Na Deoxycholate 0.25% 250uL 10% Chemical Shelf
NP-40 1% 1mL 10% Chemical Shelf
NaCl 150mM 375uL 4M Chemical Shelf
EDTA 1mM 20uL 0.5M Chemical Shelf
NaVO3 (see preparation ) 100uM 10uL 100mM Chemical Shelf
NaF 5mM 100uL 500mM Chemical Shelf
NaPPi 10 mM 1 mL 100mM Chemical Shelf
Protease Inhibitors 1 mini tablet 4C