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Quantification of Vacuolar Colocalization

Revision as of 13:42, 11 June 2010 by Davebridges (Talk | contribs) (wrote initial page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Revision as of 13:42, 11 June 2010 by Davebridges (Talk | contribs) (wrote initial page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Software

Method

  1. You Need A Merged High Resolution Image
  2. Open File in ImageJ
  3. Go To Image -> Color -> Channels Tool
  4. From the dropdown menu select Color then click OK
  5. Zoom in to see the cell
  6. Draw a Line using the Line tool. The line should start inside the cell, and cross the vacuole twice. Try to pick a representative cross section of the vacuole
  7. Select Analyse -> Plot Profile (or hit the shortcut CTRL-K)
  8. On the popup window select copy and paste the data into an excel document
  9. Move the slide bar on the bottom of the image to the green channel
  10. Select Analyse -> Plot Profile (or hit the shortcut CTRL-K)
  11. On the popup window select copy and paste the data into an excel document
Last modified on 11 June 2010, at 13:42