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Production of LentiCRISPR Viruses

147 bytes added, 16:13, 8 December 2017
Added categories
* Centrifuge at 500g for 5 minutes to pellet any cells.
* Filter supernatant through the 0.45 um. Aliquot and snap freeze in liquid nitrogen in screw capped vials.
 
[[ Category: CRISPR ]]
[[ Category: Cell Culture ]]
[[ Category: Tissue Culture ]]
[[ Category: Transfection ]]
[[ Category: Molecular Biology ]]