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Preparing an Adenoviral shRNA Clone

139 bytes added, 19:47, 13 December 2010
→‎Choosing a shRNA Sequence: updated XbaI compatible overhang
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[[Category:shRNA]][[Category:Adenovirus]][[Category:Transduction]][[Category:Knockdown]][[Category:Molecular Biology]][[Category:Cloning]]
==Choosing a shRNA Sequence==
Use oligoengine to pick a sequence, for example
<span style="color:#FF0000">GATCCTAG</span><span style="color:#00FFFF">CCC</span>GAATACCGCAATGCCTTAG<span style="color:#FFD700">TTCAAGAGA</span>CTAAGGCATTGCGGTATTC<span style="color:#00FFFF">TTTTTA</span>
Where: