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Preparing an Adenoviral shRNA Clone

102 bytes added, 18:21, 23 August 2010
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*<span style="color: #00FFFF">Spacer</span>
*<span style="color:#FFD700">Loop</span>
*In black is the target shRNA sequence, both forward and then after the loop as a reverse complement.
For the reverse primer choose the reverse complement, remove the XbaI overhang from the 3' end (the last GATC sequence) and add an AGTC sequence to the 5' end (for HindIII).
Order primers through IDT-DNA